MINORITY HIGH SCHOOL STUDENT RESEARCH APPRENTICE PROGRAM

购买流式细胞仪/细胞分选器需要资金 对于司法部至少六组调查人员而言 佛罗里达大学医学院的病理学。 这些调查的范围非常广泛，包括：a) 表面抗原、DNA含量和动力学性质的分析 人类和小鼠淋巴瘤细胞，b)检测和 糖尿病前期和内分泌中淋巴细胞抗原的定量检测 疾病，c)人类T细胞表面抗人类白细胞抗原抗体的检测 淋巴细胞和血小板，d)细胞表面的鉴定 识别元表型的标记和，e)识别和 小鼠脾B细胞表面II类分子的定量研究 淋巴细胞。尽管已经有一个流式细胞仪/细胞分选仪 存在于我们的部门，并可用于这些项目， 仪器即将过时，并将大力致力于 对与医院相关的高度优先的临床样本进行分析。这个 缺乏现成的现代化和可靠的仪器和 速度更快、性能更高的计算机正在危及当前 这些研究人员和其他研究人员的努力 对流式细胞术和细胞分选的需求日益增加。我们有 在这项技术和 组织和规划多用户实验室。我们也 有称职的人员来操作和维护 乐器。医学院的承诺和 病理学系支持我们的工作，以及 需要这种技术的资金充足的项目的数量， 确保项目的正常运作和未来的充分运作 流式细胞仪/分选设备。

Funds are requested to purchase a Flow Cytometer/Cell Sorter for at least six groups of investigators in the Department of Pathology of the College of Medicine of the University of Florida. The scope of these investigations is very broad and includes: a) analyses of surface antigens, DNA content and kinetic properties of human and murine lymphoma cells, b) detection and quantitation of lymphocyte antigens in prediabetes and endocrine diseases, c) detection of anti-HLA antibodies on human T- lymphocytes and platelets, d) identification of cell surface markers identifying metastic phenotypes and, e) identification and quantitation of Class II molecules on murine splenic B lymphocytes. Although a flow cytometer/cell sorter already exists in our department and is available for these projects, the instrument is reaching obsolescence and is heavily committed to analysis of high priority, hospital-related clinical samples. The lack of ready access to a modern and reliable instrument and a faster, high performance computer is jeopardizing the current efforts of these investigators and those of other researchers with increasing needs for flow cytometry and cell sorting. We have extensive experience with this technology and with the organization and planning of a multi-user laboratory. We also have competent personnel to operate and maintain the instruments. The commitments of the College of Medicine and the Department of Pathology to support our efforts, and the number of well funded projects that require this technology, insure the proper functioning and adequate future operation of the flow cytometry/sorting facility.