REGULATION OF PROTEIN SYNTHESIS MODULATION BY TRNA

分泌性蛋白质的合成以及分泌性蛋白质的合成点 正在使用一对 蜘蛛丝素腺体作为模型系统。 两个悬而未决 该系统的特点是腺体可以培养， 通过简单的操作， 活动可能会被关闭，然后刺激到一个 大规模生产蛋白质产品。 具有特征的 腺体，它的产物，还有模板分子，我们有， 到目前为止，已被阐明。 所有这些都发挥着调节作用， 一个平移水平，一个在伸长，另一个在读出 现在继续我们的表征，进入转录后 调控 这是合理的，因为在早期瞬变中， 在模板生产之前的大分子合成浪潮， 小核RNA的从头合成和阳性强信号 对于U1 RNA探针，在刺激的 腺体 这些结果表明，在编排的 在丝心蛋白的产生中，小RNA起着至关重要的作用。 小rna 目前正处于基因表达的聚光灯下， 在转录后处理中的作用 在真核生物中。 因此，我们的目标是表征小RNA， 特别是美国，因为他们的角色已经确立， 作为成绩单成熟者。 根据我们的初步观察， 我们将探索这些组织特异性表达的系统， 分子，并建立选择性U 基因表达并引发丝心蛋白合成。

Synthesis of a secretory protein and the points at which the process may be regulated are being investigated using a pair of spider fibroin glands as a model system. The two outstanding characteristics of the system are that the glands can be cultured in vitro, and that through simple manipulations their synthetic activity may be virtually turned off, and then stimulated into a massive production of the protein product. Having characterized the gland, its product and also the template molecule, we have, thus far, been elucidated. All of them exert their regulation on a translational level, one at elongation, the other at the readout now to move on in our characterization, into posttranscriptional regulation. This is justified, because within an early transient wave of macromolecular syntheses preceding the template production, di novo synthesis of small nuclear RNAs and positive strong signals for a U1 RNA probe have been both detected in the stimulated glands. These results indicate that within the orchestration of fibroin production, the small RNAs play a vital role. Small RNAs are currently in the limelight of gene expression because of their proven role(s) in the postranscriptional processing of transcripts in eukaryotes. We, therefore, aim to characterize the small RNAs, particularly the U ones, because of their well established roles as transcript maturators. In view of our preliminary observations, we will explore the system for tissue specific expressions of these molecules, and establish kinetic correlations between selective U gene expressions and elicited fibroin synthesis.