PROTEIN STRUCTURE/FUNCTION--A PROTEIN SEQUENCER

许多生物学功能是由蛋白质的一级结构决定的。 我们理解这些功能的能力一直受到我们能力的限制 来理解这些结构。 在过去的几年里， 开发了允许蛋白质序列分析应用于 各种重大生物学问题。 蛋白质的性质和位置 多晶型、化学修饰和底物结合 深入了解分子的功能。 一些 美国国立卫生研究院支持的乔治敦大学校园项目正在研究 免疫相关蛋白质结构与功能关系 反应，参与染色质/DNA相互作用的蛋白质， 在化疗期间发生了变化。 这些研究包括以下生物化学： (1)由HLS-D区编码的人组织相容性抗原。 （二） 聚（ADP-核糖）聚合酶，因为它涉及染色质结构和 功能 (3)与人类补体相关的眼镜蛇毒因子 组分C3 b. (4)DNA内切核酸酶。 (5)细胞内蛋白质作为 由抗肿瘤药物亚硝基脲修饰。 这些研究中的每一个都需要使用蛋白质测序仪。 这 该提案构成乔治敦大学成员的请求， 买下这台测序仪 这个测序仪将是一个重要的工具， 定义蛋白质的一级结构， 功能可以获得。

Many biologic functions are dictated by the primary structure of proteins. Our ability to understand these functions has been limited by our ability to understand these structures. Over the past few years, methods have been developed which allow the application of protein sequence analysis to a variety of major biologic questions. The nature and sites of protein polymorphism, of chemical modification and of substrate binding give insights into the way in which molecules function. A number of NIH-supported projects on the Georgetown University campus are examining the structure-function relationship for proteins involved in immune response, proteins involved in chromatin/DNA interaction and proteins alterated during chemotherapy. These studies include the biochemistry of: (1) Human histocompatibility antigens encoded by the HLS-D region. (2) Poly (ADP-ribose) polymerase as it relates to chromatin structure and function. (3) Cobra venon factor as it relates to the human complement component C3b. (4) DNA endonucleases. (5) Intracellular proteins as modified by nitrosourea, an antitumor drug. Each of these studies requires the use of a protein sequencer. This proposal constitutes a request by members of Georgetown University to purchase this sequencer. This sequencer will be an important tool in defining protein primary structure so that an understanding of protein function can be obtained.