PEPTIDE SYNTHESIZER

Beckman System 990肽合成仪的可用性将使 可能的详细化学研究的一些蛋白质有关， 癌症生物学的研究。 纤连蛋白的细胞附着位点已被鉴定， 使用合成肽对该位点进行各种修饰的效果将 阐明了细胞附着在该分子上的机制。 细胞 将确定玻连蛋白中的附着位点，并使用 通过细胞附着到这两种蛋白质上，以及其他一些蛋白质上， 包括胶原蛋白在内的蛋白质。 胶原蛋白中的位点 与纤连蛋白相互作用的蛋白质将使用合成的 缩氨酸 将使用以蛋白聚糖为模型的合成肽抗体 为了研究这些细胞外基质分子在正常人中的分布， 和恶性组织，并研究这些分子与 胶原蛋白、纤连蛋白和玻连蛋白。 此外，可预测的强 c-fos蛋白中的免疫原性位点将被合成并用于 培养抗体以研究这种蛋白质在胚胎发育中的性质和作用。 发展 最后，合成肽将用于研究蛋白质的作用机制 糖基化在不同的发展阶段。 未来的项目包括使用合成肽来研究 表皮生长因子和转化生长因子。

The availability of a Beckman System 990 peptide synthesizer will make possible the detailed chemical study of a number of proteins related to cancer biology by several investigators. The cell attachment site of fibronectin has been identified, and the effects of various modifications of that site using synthetic peptides will clarify the mechanism by which cells attach to that molecule. The cell attachment site in vitronectin will be identified, and the mechanisms used by cells to attach to these two proteins, as well as to a number of other proteins including collagens, will be investigated. The site in collagen which interacts with fibronectin will be reconstructed using synthetic peptides. Antibodies to synthetic peptides modeled after proteoglycans will be used to study the distribution of these extracellular matrix molecules in normal and malignant tissues and to study the interaction of these molecules with collagen, fibronectin, and vitronectin. Also, predictable strong immunogenic sites in the c-fos protein will be synthesized and used to raise antibodies to study the nature and role of this protein in embryonal development. Finally, synthetic peptides will be used to study the mechanisms of protein glycosylation in different stages of development. Future projects include the use of synthetic peptides to study the effects of epidermal growth factor and transforming growth factors.