FLOW CYTOMETRY RESOURSE UPGRADE

FACSIV双荧光/五参数/计算机升级 在此请求将启用位于 南阿拉巴马大学将履行以下职能 目前不适用于目前的流式细胞仪测试：1) 同时对活细胞进行DNA和细胞表面标记研究， 2)使用德克萨斯红作为替代的双色分析 藻红蛋白，3)使用德克萨斯红或 别藻蓝蛋白与FITC和FITC并列为第三代荧光色素 藻红蛋白，4)列表模式数据存储，使重新定位成为可能。 在未来日期使用不同的门控对样品进行分析 参数；同时，使同步采集成为可能 和来自五个参数的数据的存储，5)双变量 联合应用Hoechst染色进行染色体分析 色霉素A3，6)等值线图，三维等轴测，多个 直方图数据显示，7)飞行时间，8)单元格 用于克隆和杂交瘤筛选的证物。这是升级的 FACS IV/计算机资源将有助于提供新的 各种分析和制备细胞的能力 NIH资助的下列项目中的技术：1. 肿瘤胎儿抗原和单抗的特性 针对这些抗原，表达在人类和啮齿动物肿瘤上。2. 辐射诱发的44kD癌胚抗原分析 小鼠的白血病和胸腺瘤。3.分析和提纯 取自大鼠胃的壁细胞。4.细胞表面标志物 辅助性T细胞、抑制性T细胞的克隆群体分析 T细胞和T反式抑制细胞。5.表型特征 疱疹病毒特异性T细胞克隆的制备及纯化 单纯性诱导抗原。6.干扰素的设立- 耐药细胞系及其细胞受体的分析 干扰素在立克次体宿主防御研究中的应用。7. 同种异体抗原攻击对淋巴细胞亚群的影响 怀孕期间的成分与艾滋病。8.胰岛素的分离 从胰腺中分泌β细胞。9.调查 用罗丹明123研究干扰素与立克次体的相互作用。

The FACS IV dual fluorescence/fifth parameter/computer upgrade requested here will enable the Flow Cytometry Laboratory at The University of South Alabama to perform the following functions not now available with the present flow cytometry ste-up: 1) simultaneous DNA and cell surface marker studies on viable cells, 2) two-color analyses using Texas Red as an alternative to phycoerythrin, 3) three-color analyses using Texas Red or allophycocyanin as the third fluorochrome together with FITC and phycoerythrin, 4) list mode data storage, making possible the re- analysis of samples, at a future date, using different gating parameters; also, making possible the simultaneous acquisition and storage of data from five parameters, 5) bivariate chromosome analysis using the Hoechst stains together with chromomycin A3, 6) contour plot, 3-D isometric, and multiple histogram data displays, 7) time of flight, and 8) single cell deposition for cloning and hybridoma screening. This upgraded FACS IV/computer resource will be instrumental in providing new capabilities for a variety of analytical and preparative cell techniques in the following NIH funded projects: 1. The characterization of oncofetal antigens, and monoclonal antibodies to these antigens, expressed on human and rodent tumors. 2. Analysis of a 44 kD oncofetal antigen on radiation induced leukemias and thymomas in mice. 3. Analysis and purification of parietal cells from the rat stomach. 4. Cell surface marker analysis of monoclonal populations of T helper cells, T suppressor cells and T contrasuppressor cells. 5. Phenotypic characterization and purification of T cell clones specifically reactive with Herpes simplex induced antigens. 6. Establishment of interferon- resistant cell lines and analysis of cellular receptors for interferon in the study of host defense against Rickettsiae. 7. Effect of alloantigenic challenge on lymphocyte sub-set composition in pregnancy and AIDS. 8. Isolation of insulin secreting beta cells from the pancreas. 9. Investigation of interferon-rickettsiae interactions using Rhodamine 123.