High Resolution High Throughput Mass Spectrometry to Characterise Materials, Chemicals, and BioCatalysts

该提案将装备UoM新设备，使高通量高分辨率离子迁移质谱分析成为可能。这一投标将增加分析能力，并扩大我们测量各种分析物的能力。专业设备将用于测量大量新合成化学品的质量和形状。用最简单的术语来说，质谱法测量离子在真空中的运动以给出质量，离子迁移率质谱法测量离子在气体中的运动以给出形状。如果我们非常精确地测量质量，我们就能知道分子是由什么原子组成的，如果我们非常精确地测量形状，我们就能知道原子是如何排列的。因此，对于大范围的不同分子，我们将能够告诉我们已经做了什么，我们已经做了多少。PI已经制造离子迁移质谱仪并使用离子迁移质谱仪超过20年。她率先在化学的许多领域使用它，在这个提案中，我们计划做得更快，更准确。 该设备将位于曼彻斯特生物技术研究所的迈克尔巴伯合作质谱中心。它将被曼彻斯特大学的研究团队以及来自学术界和仪器的外部用户使用。我们将使用这种最先进的仪器来支持EPRSC在未来生物制造研究中心和与阿斯利康的繁荣合作伙伴关系以及材料和催化方面的其他计划中的投资。这种新的能力将包括两个质谱仪耦合到新的电离源。最先进的Cyclic IMS QTof MS仪器将为复杂混合物的分子鉴定和结构表征提供独特的增强分析能力，并提供完整的离子迁移率数据。第二个“主力”DESI接口Synapt XS仪器将提供更广泛的研究途径和更广泛的应用，并作为快速质谱分析的循环结构工作之前的初始筛选平台。电离源将使分析具有最小的样品制备，无论是直接从表面通过解吸电喷雾电离，并与微流体液滴分配，以提供一个独特的超高通量（uHTP）筛选能力。这将为一系列化学分析物提供质量和结构数据，从小分子到聚合物，从超分子化合物到生物制药。

This proposal will equip UoM new equipment that will enable high throughput high resolution ion mobility mass spectrometry analysis. This bid will increase capacity for analysis and extend our capacity to measure a wide range of analytes. Specialist equipment will be used to measure the mass and the shape of a large number of newly synthesised chemicals. In the simplest terms possible, mass spectrometry measures the movement of ions in a vacuum to give mass and ion mobility mass spectrometry measures the movement of ions in a gas to give shape. If we measure mass very accurately we can work out what atoms a molecule is made of and if we measure the shape very accurately we can work out how the atoms are arranged. So for a large range of different molecules we will be able to tell what we have made and how much we have made. The PI has been making ion mobility mass spectrometers and using ion mobility mass spectrometry for over 20 years. She has pioneered its use in many areas of chemistry and in this proposal we plan to do it even faster and with more accuracy. This equipment will be located with the Manchester Institute for Biotechnology in the Michael Barber Centre for Collaborative Mass Spectrometry. It will be used by research teams at the University of Manchester as well as by external users from both academia and instrument. We will use this state of the art instrumentation to underpin investments by EPRSC in the Future Biomanufacturing Research hub and the Prosperity Partnership with AstraZeneca as well as other programs in materials and catalysis. This new capability will consist of two mass spectrometers coupled to novel ionisation sources. A State-of-the-art Cyclic IMS QTof MS instrument will provide unique enhanced analytical capabilities for molecular identification and structural characterisation of complex mixtures complete with full ion mobility data. A second "work-horse" DESI interfaced Synapt XS instrument will provide broader research access and wider application, and act as an initial screening platform prior to Cyclic Structural work for rapid mass spectrometry analysis. The ionisation sources will enable analysis with minimal sample preparation both directly from surfaces via Desorption Electrospray ionisation and with microfluidic droplet dispensing to provide a unique ultra-high-throughput (uHTP) screening capability. This will provide mass and structural data for a range of chemical analytes from small molecules to polymers, supramolecular compounds to biopharmaceuticals.