CHARACTERIZATION OF OXYGENATED FATTY ACID METABOLITES BY CAPILLARY GC/MS

这个项目的目标是开发一种敏感和具体的 人脑脊液中前列腺素的测定 酒精中毒患者与适当使用GAS的对照 层析/质谱学。这项研究是由 因为有文献报道表明一段关系 存在于酒精对中枢神经系统的影响和 前列腺素的集中生产。尤其是在人类和 动物研究，前列腺素合成给药 乙醇给药前的抑制剂使中枢减弱 酒精对神经系统的影响。 在方法学验证完成后对样品进行分析， 所测前列腺素(PGE2、PGE1、 PGF2a、PGF1a、6-酮-PGF1a) 是定量限值(3pg/mLCSF)的两倍。比较 GC/MS和放射免疫分析方法进一步提供了 对这些结果进行验证。文献报道要高得多 正常对照组的前列腺素水平，即几十到数百 Pg/mLCSF，似乎是不正确的。对猴子的检查 脑脊液提供了阳性对照，因为几种前列腺素 在这些样品中很容易量化。

The objective of this project is to develop a sensitive and specific assay for prostaglandins in human cerebrospinal fluid (CSF) from patients with alcoholism and appropriate controls using gas chromatography / mass spectrometry. This study was initiated because of literature reports which suggested that a relationship exists between central nervous system effects of ethanol and the central production of prostaglandins. In particular, in human and animal studies, administration of prostaglandin synthesis inhibitors prior to administration of ethanol attenuated central nervous system effects of ethanol. Samples assayed after methodological validation was completed, contained, none of the measured prostaglandins (PGE2, PGE1, PGF2a, PGF1a, 6-keto-PGF1a) at a concentration more than twice the limit of quantification (3 pg/mL CSF). Comparison of GC/MS and radioimmunoassay methods provided further validation for these results. Literature reports of much higher levels of prostaglandins in normal controls, i.e., tens to hundreds of pg/mL CSF, appear to be incorrect. Examination of monkey CSF provided a positive control, since several prostaglandins were easily quantifiable in these samples.