CHARACTERIZATION OF OXYGENATED FATTY ACID METABOLITES BY CAPILLARY GC/MS

这个项目的主要目标是应用最先进的天然气 痕量分析中的色谱/质谱学方法 脂肪酸代谢产物的水平，以努力阐明分子 参与各种神经病理状态的改变。这些化合物 通常是以极低水平存在的强效制剂，如 复杂混合物的一部分，因此，其他技术通常不能 以提供有用的结果。在这项研究的第一阶段， 重点已经放在表征前列腺素水平上。 人脑脊液(CSF)。 关于GC/MS样品制备的广泛调查 分析导致了几个方法的修改，提供了 样品回收率和检出限的提高。 来自不同类别患者的脑脊液样本已经被 分析：前列腺素PGF-1a、PGF-2a和6-keto-PGF-1a是可定量的 在样本中，虽然PGE-2和PGE-2的水平通常低于 检测限值。旨在进一步改进检测的研究 其他前列腺素分析的限度和方法进展在 进步。

The principal objective of this project is to apply state-of-the-art gas chromatographic/mass spectrometric methodologies to the analysis of trace levels of fatty acid metabolites in an effort to elucidate the molecular alterations involved in various neuropathological states. These compounds are generally potent agents that are present in extremely low levels as part of a complex mixture and, therefore, other techniques often are unable to provide useful results. During the first phase of this research, emphasis has been placed on characterizing the levels of prostaglandins in human cerebrospinal fluid (CSF). Extensive investigations regarding the preparation of samples for GC/MS analysis have resulted in several methodological modifications, providing an improvement in sample recovery and the limits of detection. Cerebrospinal fluid samples from various patient classes have been analyzed; prostaglandins PGF-1a PGF-2a, and 6-keto-PGF-1a were quantifiable in the samples, while levels of PGE-2 and PGE-2 were usually below detection limits. Studies aimed at further improvements in detection limits and methods development for analysis of other prostaglandins are in progress.