EXCITATION-CONTRACTION IN ISOLATED CARDIAC CELLS

我们分离了成年大鼠和家兔的心肌细胞。 这些制剂被用于研究收缩， 电生理和生化特性，在各种 不同条件 特别是，我们已经能够确定在这些 孤立的细胞一个纵向传播的波， 在具有正常静息膜电位的细胞中， 电刺激。 这些“波”很可能代表了 肌浆中钙的自发释放现象 网织（SR）和散射光强度的原因 波动（SLIF），我们的实验室在过去的研究， 多细胞制剂。 通过我们的工作，我们初步验证了 单细胞作为模型的机制研究， 兴奋-收缩偶联，特别是自发SR钙 release. 我们最近扩大了我们的工作， 自发收缩波和受激抽搐之间的联系

We have dissociated myocardial muscle cells from adult rats and rabbits. These preparations are being used to study the contractile, electrophysiological, and biochemical characteristics in a variety of different conditions. In particular we have been able to identify in these isolated cells a longitudinally propagating wave which occurs spontaneously in cells with a normal resting membrane potential which are not electrically stimulated. These "waves" are likely to represent the phenomenon of spontaneous release of calcium from the sarcoplasmic reticulum (SR) and are the cause of the scattered light intensity fluctuations (SLIF) which our laboratory has studied in the past in multicellular preparations. With our work we have initially validated single cells as a model for the study of mechanisms of excitation-contraction coupling and in particular of spontaneous SR calcium release. We have recently expanded our work to study the interaction between spontaneous contractile waves and stimulated twitches.