AUTOCRINE MECHANISM FOR V-SIS TRANSFORMATION

使用v-sis转化的成纤维细胞系，我们能够证明 α和β血小板衍生生长因子(PDGF)受体 在细胞内的隔间中被激活。然而，表面定位 是将激活的受体偶联到有丝分裂反应所必需的。 这些发现为研究人类肿瘤提供了一个有用的模型系统。 细胞株，并了解PDGF亚型在肿瘤中可能发挥的作用 进程。另外。我们的研究表明，苏拉明这种药物 能完全消除SIS/3T3转化中的转化表型 细胞系。能够显著改变许多人类的扩散 表达α或βPDGF受体的肿瘤细胞株和A 或PDGF的B链。

Using v-sis-transformed fibroblast cell lines, we were able to demonstrate that both alpha and beta platelet-derived growth factor (PDGF) receptors are activated in an intracellular compartment. Yet a surface localization is required for coupling the activated receptors to a mitogenic response. These findings served as a useful model system to investigate human tumor cell lines and understand the role PDGF isoforms may have in the neoplastic process. Additionally. our studies indicate that the drug suramin, which can completely abrogate the transformed phenotype in sis/3T3 transformed cell lines. is able to significantly alter the proliferation of many human tumor cell lines that express either alpha or beta PDGF receptors and the A or B chain of PDGF.