INTRODUCTION OF THE HIV TAT GENE INTO LYMPHOID CELLS

为了研究人类免疫缺陷病毒（HIV）对人类免疫功能的影响达特 基因对细胞基因表达的影响，我们构建了淋巴 表达达特基因的细胞系。这是通过将达特基因 和相邻的HIV长末端重复序列（LTR）插入逆转录病毒载体中 pGV 1。将重组质粒转染psi 2细胞， 亲嗜性病毒原种，其用于感染psiAM细胞。殖民地 分离G418抗性psiAM细胞，并测定其产生。 病毒 使用具有最高病毒产量的那些菌落的上清液 感染人类T淋巴细胞系H9的细胞。G418抗性细胞 发现来源于H9的细胞系含有达特活性， 与含有LTR和CAT序列的HeLa细胞融合。而且他们 包含预测大小的LTR-tat消息。

In order to study the effect of the human immunodeficiency virus (HIV) tat gene on the expression of cellular genes, we have constructed lymphoid lines that expressed the tat gene. This was done by placing the tat gene and an adjacent HIV long terminal repeat (LTR) into the retroviral vector pGV1. The recombinant plasmid was transfected into psi2 cells to produce an ecotropic viral stock that was used to infect psiAM cells. Colonies of G418-resistant psiAM cells were isolated and assayed for the production of virus. Supernatants of those colonies with the highest virus production were used to infect cells of the human T-cell lymphoid line, H9. G418-resistant cell lines derived from H9 were found to contain tat activity as assayed by fusion with HeLa cells containing LTR and CAT sequences. Furthermore, they contained an LTR-tat message of the predicted size.