PRENATAL DIAGNOSIS USING FETAL CELLS OBTAINED FROM MATER

本项目的目的是开发一种安全、相对 廉价、准确、无创的产前检查技术 分离母体胎儿细胞诊断遗传性疾病 妊娠前三个月的外周循环。 分离将通过流式细胞仪完成， 滋养细胞抗体和人类白细胞抗原抗体。方法将是 为培养胎儿细胞而开发的。胎儿非整倍体和 多倍体将通过测量胎儿细胞DNA来检测 流式细胞术(DNA索引)是由 调查人员。这些技术可用于产前检查。 细胞遗传学、生化或DNA异常的诊断。

The purpose of this project is to develop a safe, relatively inexpensive, accurate and non-invasive technique for the prenatal diagnosis of genetic disorders by isolating fetal cells in maternal peripheral circulation during the first trimester of pregnancy. Separation will be accomplished by flow cytometry with trophoblast antibodies and HLA antibodies. Methods will be developed for culturing fetal cells. Fetal aneuploidy and polyploidy will be detected by measuring fetal cellular DNA with flow cytometry (DNA indexing), a sensitive method developed by the investigators. These techniques can be used for the prenatal diagnosis of cytogenetic, biochemical or DNA abnormalities.