Development of noninvasive prenatal diagnosis using nucleated erythrocyte in maternal blood

已经作出了相当大的努力，以发展无创产前诊断程序使用有核红细胞（nrbc）在母体循环。由于在母体血液中循环的nrbc数量有限，因此需要进行细胞富集和高灵敏度单细胞分析的程序。我们开发了优化的nrbc分离方法，包括基于凝集素的方法和自动图像分析方法。此外，我们优化了分离程序的每一步，以最大限度地提高回收率。接下来，我们评估了日本对无创产前诊断（NIPD）的态度。结果显示，孕妇更重视NIPD检测的安全性和非侵入性，而医疗专业人员则认为诊断的准确性和可靠性是最重要的。卫生专业人员指出，可能经常在孕妇没有充分了解或咨询的情况下进行检测。

Considerable effort has been made to develop non-invasive prenatal diagnostic procedures using nucleated erythrocytes (NRBCs) within the maternal circulation. Since a limited number of NRBCs circulate within maternal blood, procedures to enrich the cells and enable single cell analysis with high sensitivity are required. We have developed the optimized separation methods of NRBCs, including a lectin-based method and autoimage analyzing. Furthermore, we optimized every step of separation procedures to maximize the recovery.Next, we assessed the attitudes toward non-invasive prenatal diagnosis (NIPD) in Japan. As results, we revealed that pregnant women place importance on the safety and non-invasiveness of the NIPD tests, whereas medical professionals consider the diagnostic accuracy and reliability of the test to be the most important. Health professionals pointed out that the tests might be frequently performed without the pregnant women having adequate knowledge or counseling.