PRENATAL DIAGNOSIS USING FETAL CELLS FROM MATERNAL BLOOD

该项目的目的是开发一种安全、相对 廉价、准确和非侵入性的产前技术， 通过分离母体中的胎儿细胞诊断遗传性疾病 外周循环在怀孕的头三个月。 分离将通过流式细胞术完成， 滋养层抗体和HLA抗体。 方法将 用于培养胎儿细胞 胎儿非整倍体和 将通过用以下方法测量胎儿细胞DNA来检测多倍性： 流式细胞术（DNA索引），一种由 调查人员 这些技术可用于产前 细胞遗传学、生物化学或DNA异常的诊断。

The purpose of this project is to develop a safe, relatively inexpensive, accurate and non-invasive technique for the prenatal diagnosis of genetic disorders by isolating fetal cells in maternal peripheral circulation during the first trimester of pregnancy. Separation will be accomplished by flow cytometry with trophoblast antibodies and HLA antibodies. Methods will be developed for culturing fetal cells. Fetal aneuploidy and polyploidy will be detected by measuring fetal cellular DNA with flow cytometry (DNA indexing), a sensitive method developed by the investigators. These techniques can be used for the prenatal diagnosis of cytogenetic, biochemical or DNA abnormalities.