DEVELOPMENT OF CELL LINES EXPRESSING OXYGEN GENERATORS

拟议研究的目的是在体外培养哺乳动物细胞， 其过量产生氧自由基，因此可以用作模型 研究氧自由基效应的系统和保护方法 抗氧自由基损伤。 这些细胞系将由 构建含有编码氧自由基的cDNA的载体 产生酶，然后将载体导入人体， 淋巴母细胞样细胞系AHH-1。 这些cDNA将在 可调节的促进剂，使得氧自由基产生的水平可以 被监管。 这些细胞系将用于研究 氧自由基对各种细胞过程的影响，包括DNA损伤和 识别防止氧自由基损害的试剂。

The object of the proposed research is to develop mammalian cells in vitro, which overproduce oxygen radicals and therefore can be used as model systems for studying oxygen radical effects and methods of protecting against oxygen radical damages. The cell lines will be developed by constructing vectors containing cDNAs which code for oxygen radical producing enzymes and then transfecting the vectors into the human lymphoblastoid cell line AHH-1. The cDNAs will be under the control of regulatable promoters so that the levels of oxygen radical production can be regulated. These cell lines will then be used to study the effects of oxygen radicals on various cellular processes, including DNA damage and to identify agents which protect against oxygen radical damage.