IDENTIFICATION AND CHARACTERIZATION OF A NOVAL PEPTIDE OSTEOCLASTPOIETIC FACTOR

我们已经确定了一种新的因素，它刺激了 破骨细胞，由人类肿瘤以及由 用刀豆蛋白A刺激的白细胞，我们称之为 这种多肽是破骨细胞生成因子(OPF)。我们已经得到了氨基酸 对该多肽进行了序列测定，发现它是新的，与 其他已知的生长调节因子、白介素类和菌落 刺激因素。我们发现一种合成肽 与这种氨基酸序列相对应的是模拟生物效应， 我们已经生产出了单抗和多克隆抗体 中和原来的生物活性。在这个项目中，我们计划 致： 1.克隆基因并表达重组分子。 2.确定OPF在正常骨重建和疾病中的作用 与骨吸收增加相关的状态。 新型细胞因子是一种强有力的刺激因子 破骨细胞性骨吸收可能导致对 破骨细胞功能的正常控制及其病理生理学 与骨骼破坏相关的疾病状态。

We have identified a novel factor which stimulates the formation of osteoclasts and which is produced by a human tumor as well as by leukocytes which have been stimulated with concanavalin A. We have called this peptide osteoclastpoietic factor (OPF). We have obtained amino acid sequence on this peptide and found that it is novel and distinct from other known growth regulatory factors, interleukins, and colony stimulating factors. We have found that a synthetic peptide which corresponds to this amino acid sequence mimics the biological effects, and we have produced both monoclonal and polyclonal antibodies which neutralize the original biological activity. In this project, we plan to: 1. Clone the gene and express the recombinant molecule. 2. Determine the role of OPF in normal bone remodeling and in disease states associated with increased bone resorption. Characterization of novel cytokines which are powerful stimulators of osteoclastic bone resorption is likely to lead to new insights in the normal control of osteoclast function and in the pathophysiology of disease states associated with bone destruction.