SURFACE DEPENDENT REACTIONS IN THROMBOSIS & THROMBOLYSIS

本计划项目的总体目标是了解流程如何 凝血、血栓形成和纤维蛋白溶解的启动、调节和 终止 这一目标包括获得对以下方面的基本了解： 这些过程之间的相互关系，并最终获得 合理操作它们进行诊断和治疗的方法 目的 一个越来越清晰的概念是， 凝血和纤维蛋白溶解的大部分调节通过 大分子复合物在膜和纤维蛋白表面的组装。 因此，当我们研究个体的功能特性时， 分子，我们的重点是那些特性，属于 调节分子的功能。 这种方法包括研究 利用模型系统的大分子组装体， 囊泡以及血小板、纤维蛋白和内皮细胞。 有 也有研究旨在确定维生素K的这些特性， 依赖性凝血因子，其导致表达的调节， 羧化水平和膜结合现象。 的 四个项目的组合和该计划的两个核心将导致 详细研究了表面依赖反应， 血栓形成和血栓溶解，然而，所发现的原理 在生物学中无处不在的应用。

The overall goal of this Program Project is to understand how the processes of coagulation, thrombosis and fibrinolysis are initiated, regulated and terminated. This goal includes obtaining a fundamental understanding of the interrelationships between these processes, and ultimately obtaining the means to rationally manipulate them for diagnostic and therapeutic purposes. One of the concepts that has become increasingly clear is that much of the regulation of coagulation and fibrinolysis takes place through the assembly of macromolecular complexes on membrane and fibrin surfaces. Therefore, while we will study the functional properties of individual molecules, our emphasis is on those characteristics which pertain to the regulation of that molecule's function. This approach includes studies of macromolecular assemblies utilizing model systems such as synthetic vesicles, as well as platelets, fibrin and endothelial cells. There are also studies aimed at determining those characteristics of the vitamin K- dependent coagulation factors which result in regulation of expression at the level of carboxylation and thus the membrane binding phenomenon. The composite of the four projects and the two cores of this program will lead to detailed investigations of surface dependent reactions specific in thrombosis and thrombolysis, however, the principles discovered have ubiquitous applications in biology.