MOLECULAR GENETIC ANALYSIS OF FELINE CELLULAR GENES--A COMPARATIVE APPROACH

在人类基因组的技术和迫切性的推动下 倡议，强调在模式物种的比较基因图谱， 近年来有所增加。 除了协调比较 通过对哺乳动物的基因分析，我们的实验室已经扩展了一个强大的 为家猫提供全面基因图谱的倡议 （Felis卡图斯）。 该图谱由两个位点类别组成：I型， 用于比较参照的进化保守编码基因座 和II型基因座，高变微卫星和小卫星基因座， 对疾病系谱分析有较高价值 表型 我们已经指定了321个锚定参考类型I 基因座进行比较推理，并将这些放在猫的连锁图谱上 利用F.卡图斯和锯尾龙 孟加拉虎，亚洲豹猫。到目前为止，超过80个种间杂交 人工授精后出生的后代;其中， 健康的雌性正在成熟，37个回交后代已经 得到了 从M13卫星中选出了300多个微型卫星类别， 噬菌体文库 对这些位点进行测序和聚合酶链反应 设计并合成用于多态性筛选的引物。 方法 猫中期染色体原位杂交 已经尝试并改进了标记的分子克隆。 程序 使用人类染色体文库进行染色体“绘画”，即， 食肉动物之间同源染色体片段的可视化， 灵长类动物，正在进行中。 Ateles（蜘蛛）的初步物理图 猴）也已经使用体细胞杂交体构建并对齐 人类和其他灵长类动物的基因图谱。

Driven by the technology and imperativeness of the human genome initiative, emphasis in comparative gene mapping in model species has increased in recent years. In addition to coordination of comparative gene analysis across mammals, our laboratory has extended a strong initiative to provide a comprehensive gene map for the domestic cat (Felis catus). The map consists of two locus categories: Type I, evolutionary conserved coding gene loci of use for comparative reference and Type II loci, hypervariable micro- and minisatellite loci with high polymorphism content and of high value for pedigree analysis of disease phenotypes. We have designated a list of 321 anchored reference Type I loci for comparative inference and are placing these on a cat linkage map using interspecies backcrosses between F. catus and Prionailurus bengalensis, the Asian leopard cat. To date, over 80 interspecies hybrid offspring have been born following artificial insemination; of these, 12 healthy females are maturing and 37 backcross offspring have been obtained. Over 300 microsatellite classes have been selected from an M13 phage library. The loci were sequenced and polymerase chain reaction primers designed and synthesized for polymorphism screens. Methods for in situ hybridization of feline metaphase chromosomes using biotin- labeled molecular clones have been attempted and improved. Procedures using human chromosome libraries for chromosome "painting," i.e., visualization of homologous chromosome segments between carnivores and primates, are in progress. A preliminary physical map of Ateles (spider monkey) has also been constructed using somatic cell hybrids and aligned with the human and other primate gene maps.