REAL-TIME HIGH-PERFORMANCE CONFOCAL LASER SCANNING OPTICAL MICROSCOPE

一种非移动部件、每秒30帧的激光扫描共焦装置 反射光和荧光显微镜已经研制成功。 声光光束偏转器，由全数字电子设备控制， 在512行隔行扫描的栅格中移动激光束。光线进入一个 倒置显微镜通过后面的荧光光口，是 通过显微镜物镜在物体上成像。反射的或 荧光光通过物镜返回，通过相机射出 端口，并被成像到像分光管的光电阴极上 (IDT)。共焦度是通过对IDT孔径进行栅格扫描来提供的 与入射到地球表面的激光的相干图像一致 对象。各种类型的实时、反射和荧光图像 已经产生了大量的生物物体。改进的电子设备和 简化的光学系统克服了通常的色散问题 声光使激光波长能够容易地改变， 从而产生了一个更加灵活的系统。

A no-moving-parts, 30-frames-per-second, laser beam scanning confocal reflected light and fluorescence microscope has been developed. Acousto-optic beam deflectors, controlled by all-digital electronics, move a laser beam in a 512-line, interlaced raster. The light enters an inverted microscope through the rear epifluorescence light port and is imaged at the object by the microscope objective. Reflected or fluorescent light returns through the objective, exits through the camera port, and is imaged onto the photocathode of an image dissector tube (IDT). Confocality is provided by raster-scanning the IDT aperture coincident with the congruent image of the laser beam incident on the object. Real-time, reflected, and fluorescent light images of a variety of biological objects have been produced. Improved electronics and simplified optics overcome the usual dispersive problem with acousto-optics to enable the laser wavelength to be easily changed, resulting in a much more flexible system.