DEVELOPMENT OF AN ELISA TEST FOR THE DETECTION OF PNEUMOCYSTIS CARINII

肺孢子虫感染的诊断可以利用多种 不同的染色程序。 这些方法可能相当敏感 但是需要高水平的复杂性能， 测试的解释。 此外，这些污渍通常 耗时，包括执行时间和读取染色的时间 污迹 ELISA方法目前已广泛应用于诊断中 传染性病原体。 方法已被简化，以便测试 需要几分钟到几个小时的时间来执行， 因为人们所寻找的只是反应中的颜色变化。 我们有4种不同的单克隆抗体可用于追求这一点 项目 将尝试使其中之一适用于ELISA 程序，虽然这将需要大量的开发工作， 为了定义最佳程序以获得最佳灵敏度，灵敏度， 快速和简单。

The diagnosis of Pneumocystis infection can be made utilizing a variety of different staining procedures. These methods can be quite sensitive but require a high level of sophistication in the performance and interpretation of the tests. In addition, these stains are generally time consuming, including time to perform and time to read the stained smears. ELISA methodology is being used extensively now in the diagnosis of infectious agents. Methods have been simplified so that the test takes from minutes to a few hours to perform and requires no skill at interpretation since all one looks for is a color change in the reaction. We have 4 different monoclonal antibodies available to pursue this project. Attempts will be made to adapt one of these to an ELISA procedure, although it will take extensive developmental work in order to define the best procedure to obtain optimal sensitivity, sensitivity, rapidity and simplicity.