CELLULAR & MOLECULAR STUDIES OF RENAL TRANSPORT

研究人员将研究细胞和分子的运输 Na、K、Cl和H等单个离子穿过单个离子的操作 肾小管细胞膜。此外，通过什么机制(S) 跨上皮液的运动与溶质的运动相耦合 探索过了。分离的小管，肾细胞培养，单肾小管细胞， 单细胞膜、肾膜制剂以及纯化 将使用运输蛋白。 形态、生化、细胞生物学和分子生物学 形态计量学、免疫细胞化学和分子探针技术 (针对各种膜蛋白的抗体)将补充功能 评估个别传输参数(离子泵、传输器和 单通道)。核心设施将为集团提供重要支持 研究努力和广泛的合作研究。 研究项目将评估肾脏的基本特性。 运输过程和错乱的体液平衡。结果是 我们的研究将对了解肾脏疾病(液体和 酸碱代谢)与液体平衡的合理治疗 精神错乱。

The investigators will study the cellular and molecular transport operations by which individual ions such as Na, K, Cl and H traverse single membranes of tubule cells. In addition, the mechanism(s) by which transepithelial fluid movement is coupled to that of solutes will be explored. Isolated tubules, renal cell cultures, single tubule cells, single cell membranes, renal membrane preparations as well as purified transport proteins will be used. Morphological, biochemical, cell biological and molecular biological techniques including morphometry, immunocytochemistry and molecular probes (antibodies to various membrane proteins) will complement the functional assessment of individual transport parameters (ion pumps, transporters and single channels). Core facilities will importantly support the group research effort and a wide range of collaborative studies. The research projects will evaluate fundamental properties of renal transport processes and deranged body fluid homeostasis. The results of our studies will have an impact on understanding renal disease (fluid and acid-base metabolism) and the rational treatment of fluid balance disorders.