BIOLOGY OF BRAIN TUMORS

加州大学洛杉矶分校脑瘤研究中心的使命是建立一个 多学科学术努力研究脑瘤和开发 新的基于生物的治疗方法。该团队由以下人员组成 神经外科，神经学，分子生物学的研究人员和临床医生， 免疫学和生物技术。该中心的主题是生物 星形细胞脑瘤的分子水平特征。新的 脑肿瘤生物学知识将引领这项计划走向新的临床 接近了。尤其是基因、受体和发育 脑肿瘤特有的受调控蛋白将被开发出来 反义和其他生物学方法。 一系列高度集中的多学科研究项目将 从事分子生物学、免疫学和生物技术方面的研究。这个 该项目将得到脑组织/临床数据库的支持。项目 #1将准备实体肿瘤和细胞系的分子图谱 (主要通过Northern杂交分析)使用癌基因和编码基因 对于中间丝蛋白发育调节的神经 标志物和重要的受体。这一方法将得到以下补充 亚主动杂交技术寻找新的标记。项目#2 将专注于体外肿瘤和早期传代中的基因表达 文化。项目3以激动人心的初步数据为基础使用 外周苯二氮卓类受体的反义寡核苷酸 将阐明基因的表达/缺失如何影响肿瘤细胞 生死存亡。类似的分析将对识别出的新基因进行。 通过从项目#1减去杂交。项目#4将研究 免疫肿瘤细胞网络。细胞炎性反应的特点 表型和细胞因子反应将被定义并相互作用 我们将研究肿瘤与炎症反应之间的关系。 项目5将开发并应用于临床实践的技术 将在术中确定大脑和肿瘤的功能区。我们 将通过以下方式完成我们的使命： -建立和维持一个组织结构，该结构将 促进和加强多学科、跨部门和 促进研究、传播研究成果和培训的跨机构努力 脑瘤专家。 -评估中心的进展，并开始识别和吸引其他人 资金来源以确保加州大学洛杉矶分校脑肿瘤的活动 研究中心可以扩展到超过三年的资金 可行性拨款。

The mission of the UCLA Brain Tumor Research Center is to establish a multidisciplinary academic effort to study brain tumors and to develop new biologically based treatment approaches. The team consists of researchers and clinicians in neurosurgery, neurology, molecular biology, immunology and biotechnology. The theme of the Center is the biological characterization of astrocytic brain tumors at the molecular level. New knowledge of brain tumor biology will lead this program to novel clinical approaches. In particular, genes, receptors, and developmentally regulated proteins unique to brain tumors will be exploited using antisense and other biological approaches. A series of highly focused multidisciplinary research projects will be conducted in molecular biology, immunology and biotechnology. The project will be supported by a Brain Tissue/Clinical Data Bank. Project #1 will prepare molecular profiles of solid tumors and cell lines (primarily by Northern blot analysis) using =oncogenes and genes coding for intermediate filament proteins developmentally regulated neural markers, and important receptors. This approach will be complemented by subractive hybridization technology to find novel markers. Project #2 will focus on gene expression in both the ex vivo tumor and early passage cultures. Project #3 building on exciting preliminary data using antisense oligodeoxynucleotides to the peripheral benzodiazepine receptor will elucidate how the expression/absence of genes influences tumor cell survival. Similar analyses will be carried out for new genes identified by substractive hybridization from project #1. Project #4 will study immune-tumor cell networks. The character of the cellular inflammatory phenotype and cytokine response will be defined and mutual interactive effects between the tumor and inflammatory response will be examined. Project #5 will develop and bring to clinical practice technology which will identify functional areas of brain and tumor intraoperatively. We will accomplish our mission by: -Establishing and maintaining an organizational structure which will facilitate and enhance multidisciplinary, interdepartmental, and interagency efforts to promote research, disseminate findings, and train specialists in brain tumors. -Evaluating Center progress and begin to identify and attract other funding resources to assure that the activities of the UCLA Brain Tumor Research Center can expand beyond the three-year funding of the feasibility grant.