SEROLOGICAL RESPONSE TO BORDETELLA PERTUSSIS ANTIGENS

人类对几种包膜相关蛋白的血清学反应 用免疫印迹法检测百日咳杆菌腺苷环化酶毒素 技巧。慢性病患者血清识别的抗原 培养确认的百日咳以及接种了疫苗的婴儿的血清 三剂常规全细胞百日咳疫苗包括63,000份 DA蛋白被证明与一种抗原性相关 分枝杆菌热休克蛋白，在结构上与 伴侣蛋白家族，参与蛋白质折叠和组装。 百日咳杆菌脂低聚糖也能被抗体识别 这些血清中的某些。29,000个Da物种与来自中国的血清反应 恢复期的个体，而91,000 Da的物种与血清发生反应 来自接种疫苗的个人。腺苷环化酶毒素的抗体是 在被诊断为百日咳的人的血清中很常见，然而这些 在接种三剂疫苗的儿童中很少发现抗体。 DTP疫苗。新生儿(脐带血)和相应的血清 对孕妇血清进行腺苷波氏杆菌抗体检测。 循环酶。类似水平的抗原特异性抗体(Ig G同型)是 在给定的新生儿-母体对的每个血清样本中发现 腺苷环化酶的抗体被动地通过 胎盘。这些抗体可能是由以前的 母亲接触百日咳杆菌或为母亲接种疫苗 百日咳疫苗，因为目前正在使用的所有四种百日咳疫苗 在美国，它们能够在小鼠身上引发与之反应的抗体 腺苷环化酶。或者，这些抗体可能是由 母亲暴露于交叉反应抗原，如大肠杆菌 因为所有的新生儿血清中都含有抗体反应性 用这种抗原。腺苷环化酶抗体在此之前 能被动保护小鼠免受百日咳杆菌的攻击。 因此存在这样一种可能性，即这些抗体可能有助于 新生儿对抗百日咳。

The human serological response to several envelope-associated proteins and adenylate cyclase toxin of B. pertussis was examined using immunoblot techniques. Antigens recognized by sera from individuals with culture-confirmed pertussis as well as by sera from infants immunized with three doses of conventional whole-cell pertussis vaccine included a 63,000 Da protein that was shown to be antigenically related to a mycobacterial heat-shock protein and structurally related to the chaperonin family of proteins involved in protein folding and assembly. B. pertussis lipooligosaccharide was also recognized by antibodies in certain of these sera. A 29,000 Da species reacted with sera from convalescent individuals whereas a 91,000 Da species reacted with sera from vaccinated individuals. Antibodies to adenylate cyclase toxin were common in sera from individuals diagnosed with pertussis, however these antibodies were rarely found in children who had received three doses of DTP vaccine. Sera from neonates (cord blood) and corresponding maternal sera were examined for antibodies to Bordetella adenylate cyclase. Similar levels of antigen specific antibodies (IgG isotype) were found in each serum sample of a given neonate-maternal pair suggesting that antibodies to adenylate cyclase are passively transferred through the placenta. These antibodies may have been elicited by previous exposure of the mothers to B. pertussis or by vaccination of the mother with pertussis vaccine since all four pertussis vaccines currently in use in the US were capable of eliciting in mice antibodies reactive with adenylate cyclase. Alternatively, these antibodies may have been elicited by exposure of mothers to cross-reactive antigens such as E. coli a-hemolysin since all of the neonatal sera contained antibodies reactive with this antigen. Antibodies to adenylate cyclase have been previously shown to passively protect mice against challenge with B. pertussis. Therefore the possibility exists that these antibodies may help protect the newborn child against pertussis.