DETERMINATION OF URUSHIOL IN ALLERGENIC EXTRACTS--URUSHIOL STABILITY STUDIES

毒藤等变应原提取物中的活性成分 毒柏属植物通常被指定为 “漆酚”，一种五或七十八烯基儿茶酚的混合物。提取物 目前可用于口服或注射的药物均为 在植物油中，例如杏仁油、玉米油或橄榄油。漆酚化合物可能 氧化成邻苯二酚，能够经受迈克尔- 与亲核化合物的加成反应。其他上的烯烃站点 漆酚分子可能起到亲核剂的作用，导致 溶液中漆酚的齐聚作用。这项工作的目标 项目是：a)开发定量分析的方法 漆酚，包括个别化合物的形态形成；b)开发 从植物油中分离漆酚的样品制备技术 基准表；c)制定方法学，以确定氧化和 低聚漆酚降解产物；d)相关漆酚 浓度、溶液类型、储存等因素对降解的影响 条件。用气相色谱对漆酚样品进行了形态分析和定量 三甲基硅基衍生物的极性固定相色谱 阶段，OV-225。对该方法进行了验证研究。 采用硅胶色谱柱的正相高效液相色谱方法 以及一种毛细管气相色谱程序 将提供更好的个别化合物的分辨率。程序： 高纯度漆酚的分离(大于100微克/毫升) 从植物油中提取的浓缩物已经得到验证，开发一种 固相萃取法分离低(少)漆酚 浓度超过10微克/毫升)正在进行中。初步研究 凝胶渗透法测定漆酚低聚物的形态 已经进行了层析。

The active constituents in allergenic extracts of Poison Ivy and other plants of the genus toxicodendron have been generically designated as "urushiol", a mixture of penta- or heptadecenyl catechols. Extracts currently available for oral administration or injection are formulated in plant oils, e.g. almond, corn or olive oil. Urushiol compounds may oxidize to orthoquinones which are capable of undergoing Michael- addition reactions with nucleophilic compounds. Olefinic sites on other urushiol molecules may function as nucleophiles, resulting in oligomerization of urushiols present in solution. Objectives of this project are: a) to develop methods for the quantitative analysis of urushiol, including speciation of individual compounds; b) to develop sample preparation techniques to isolate urushiol from plant oil matrices; c) to develop methodologies to determine oxidized and oligomerized urushiol degradation products; d) to correlate urushiol degradation with factors of concentration, solution type and storage conditions. Urushiol samples have been speciated and quantitated by gas chromatography of trimethylsilyl derivatives using a polar stationary phase, OV-225. Validation studies have been performed for this method. A normal phase HPLC method utilizing a silica column is to be investigated as well as a capillary gas chromatographic procedure that would provide better resolution of individual compounds. Procedures for the isolation of urushiol at high (greater than 100 microgram/ml) concentrations from plant oils have been validated, development of a solid phase extraction procedure for isolation of urushiol at low (less than 10 microgram/ml) concentrations is in progress. Initial studies involving speciation of urushiol oligomers by gel permeation chromatography have been performed.