METABOLIC STUDIES OF OPPORTUNISTIC FUNGI AND PROTOZOA

本申请代表了一种生物学方法来开发新的 获得性免疫系统中机会性感染的治疗形式 缺乏综合征（艾滋病）。 该方法涉及五个项目， 专注于机会性真菌的代谢和生物化学研究， 原生动物，这将提供重要的信息， 这是一个新的需求，并导致新的治疗目标的发展。 项目1涉及无菌培养系统的开发， 卡氏肺孢子虫。 项目2侧重于运输分析 卡氏肺吸虫脂质代谢的研究 项目3涉及研究 刚地弓形虫和卡氏疟原虫的嘌呤代谢。 项目4是 与新生隐球菌中D-甘露醇代谢有关。 项目 5涉及细胞色素P450羊毛甾醇14-α-脱甲基酶的研究 和C.新人类 的 应用程序强调密切协作和沟通， 研究人员，以便获得有关一个生物体的知识可以应用于 其他机会性病原体。

This application represents a biological approach to the development of new forms of treatment of opportunistic infections in the acquired immune deficiency syndrome (AIDS). The approach involves five projects which focus on metabolic and biochemical studies of opportunistic fungi and protozoa which will provide important information about their nutrient requirements and lead to the development of new therapeutic targets. Project 1 deals with the development of an axenic culture system for Pneumocystis carinii. Project 2 focuses on the analysis of the transport and metabolism of the lipids of P. carinii. Project 3 involves studies of purine metabolism of Toxoplasma gondii and P. carinii. Project 4 is concerned with D-mannitol metabolism in Cryptococcus neoformans. Project 5 deals with studies of the cytochrome P450 lanosterol 14-alpha-demethylase and NADPH-cytochrome P450 reductase genes of C. neoformans. The application emphasizes close collaboration and communication among investigators so that knowledge gained about one organism can be applied to other opportunistic pathogens.