GADD153, A GENE EXPRESSED IN RESPONSE TO DNA DAMAGE AND GROWTH ARREST

分子反应基因也由生长停滞诱导， 氧化应激 很可能gadd 153基因产物在 在细胞对逆境的反应中， 增殖 对DNA损伤的反应：DNA损伤后gadd 153基因表达的诱导 损伤主要在转录水平上受到控制。 我们有 在启动子区域内描绘的序列对DNA重要， 损伤介导的GADD 153转录激活，并已鉴定 gadd 153的几个特征使其在DNA损伤中独一无二， 诱导基因：通过DNA损伤激活gadd 153转录 在启动子区域内需要多个元件， C敏感。 生长停滞诱导：使用gadd 153启动子-CAT报告基因 已经表明培养基耗尽激活GADD 153转录的同时 生长停滞。 启动子区的初始缺失分析 表明积极和消极的调节因素都很重要 在控制基因的表达与生长停滞。

The molecular response gene is also induced by growth arrest and with oxidative stress. It is likely that the gadd153 gene product plays a role in the cellular response to adversity, perhaps in controlling cell proliferation. Response to DNA damage: Induction of gadd153 gene expression following DNA damage is controlled primarily at the level of transcription. We have delineated sequences within the promoter region which are important for DNA damage-mediated activation of gadd153 transcription and have identified several features of gadd153 which make it unique among the DNA damage inducible genes: Activation of gadd153 transcription by DNA damage requires multiple elements within the promoter region and is protein kinase C sensitive. Induction by Growth Arrest: Using a gadd153 promoter-CAT reporter genewe have shown that medium depletion activates gadd153 transcription concurrent with growth arrest. Initial deletion analysis of the promoter region suggests that both positive and negative regulatory elements are important in controlling the expression of the gene with growth arrest.