STUDIES ON THE STRUCTURE AND FUNCTION OF HEAT-LABILE TOXIN FROM BORDETELLA

不耐热毒素(HLT)是由波氏杆菌产生的一种强效毒素。 这种毒素会导致组织损伤，并可能导致观察到的损伤。 在感染之后。只有另一种毒素，气管细胞毒素，是已知的 由波尔德氏菌属的所有四个种产生。由于不同的 虽然在不同的宿主上，不同的物种会产生相似的疾病，但HLT可能会发挥作用 在生物体的发病机制中起着重要作用。我们已经发起了一项 程序来检查这种毒素的结构和功能。 建立了HLT的细胞培养方法。在这项试验中， 测定HLT对血管平滑肌细胞收缩的诱导作用。这个 通过这种方法获得的结果，允许检测到少量的AS 0.6 ng/ml的毒素，与体内检测结果具有良好的相关性 化验。 HLT对豚鼠皮损的诱导能力被发现是 被脂质抑制的。长链脂肪酸对HLT有抑制作用； 然而，脂肪醇、中性脂、磷脂、胆固醇和 前列腺素对HLT的作用无明显影响。这些数据表明 HLT在动物身上引起皮肤损伤的能力可能取决于 部分，对皮肤层中游离脂肪酸含量的影响。

Heat-labile toxin (HLT) is a potent toxin produced by Bordetella species. This toxin induces tissue damage and may contribute to damage observed after infection. Only one other toxin, tracheal cytotoxin, is known to be produced by all four species in the genus Bordetella. Since the different species produce similar diseases albeit in different hosts, HLT may play an important role in the pathogenesis of the organisms. We have initiated a program to examine the structure and function of this toxin. A cell culture assay for HLT was developed. In this assay, the ability of HLT to induce contraction of vascular smooth muscle cells is measured. The results obtained by this method, which allows for detection of a little as 0.6 ng/ml of the toxin, correlated well with those obtained with in vivo assays. The ability of HLT to induce skin lesions in guinea pigs was found to be inhibited by lipids. Long-chain fatty acids were found to inhibit HLT; however, fatty alcohols, neutral lipids, phospholipids, cholesterol, and prostaglandin had no measurable effect on HLT action. These data suggest that the ability of HLT to induce skin lesions in animals may depend, in part, on the free fatty acid content of the skin layer.