METABOLIC STUDIES OF OPPORTUNISTIC FUNGI AND PROTOZOA

这一应用代表了一种生物学方法来开发新的 获得性免疫中机会性感染的治疗形式 虚证(艾滋病)。该方法涉及五个项目，这些项目 关注机会性真菌的代谢和生化研究 原生动物，这将提供关于它们营养的重要信息 要求，并导致新的治疗靶点的开发。 项目1涉及开发无菌培养系统，以 卡氏肺孢子虫。项目2侧重于对运输的分析 和卡氏肺孢子虫的脂类代谢。项目3涉及以下研究 弓形虫和卡氏弓形虫的嘌呤代谢。项目4是 新生隐球菌中D-甘露醇代谢的研究。项目 5细胞色素P450羊毛甾醇14-α-脱甲基酶的研究 和新生葡萄球菌NADPH-细胞色素P450还原酶基因。这个 应用程序强调相互之间的密切协作和沟通 研究人员，以便将获得的关于一种生物的知识应用于 其他机会性病原体。

This application represents a biological approach to the development of new forms of treatment of opportunistic infections in the acquired immune deficiency syndrome (AIDS). The approach involves five projects which focus on metabolic and biochemical studies of opportunistic fungi and protozoa which will provide important information about their nutrient requirements and lead to the development of new therapeutic targets. Project 1 deals with the development of an axenic culture system for Pneumocystis carinii. Project 2 focuses on the analysis of the transport and metabolism of the lipids of P. carinii. Project 3 involves studies of purine metabolism of Toxoplasma gondii and P. carinii. Project 4 is concerned with D-mannitol metabolism in Cryptococcus neoformans. Project 5 deals with studies of the cytochrome P450 lanosterol 14-alpha-demethylase and NADPH-cytochrome P450 reductase genes of C. neoformans. The application emphasizes close collaboration and communication among investigators so that knowledge gained about one organism can be applied to other opportunistic pathogens.