OPTIMAL METHODOLOGY FOR DETECTION OF CMV IN CLINICAL LABORATORY

108例咽及尿常规病毒培养结果分析 培养和壳瓶显示壳瓶的灵敏度为84 法在这项研究中，我们还评估了治疗的有效性。 装有地塞米松/DMSO的外壳小瓶。我们最初发现治疗 DEX/DMSO增加了观察到的病毒灶的数量， 单独用DEX治疗是不够的。几种不同的单克隆 测试抗体染色剂以确定哪种染色剂最敏感 使用.初步比较表明，杜邦单克隆IFA优于 Ortho DFA背景染色过多。我们随后比较了 杜邦IFA与Syva IFA。后者也有非特异性的问题， 这是一种污染，已经从市场上消失了。

A comparison of 108 throat and urine cultures done by conventional viral culture and by shell vial showed 84% sensitivity of the shell vial technique. During this study we also evaluated the usefulness of treating the shell vials with Dexamethasone/DMSO. We initially found that treatment with DEX/DMSO increased the number of viral foci observed and that treatment with DEX alone was insufficient. Several different monoclonal antibody stains were tested to determine which stain was the most sensitive to use. Initial comparison showed the Dupont monoclonal IFA better than the Ortho DFA which had too much background staining. We subsequently compared the Dupont IFA with Syva IFA. The latter also had problems with nonspecific staining and has since been taken off the market.