EXPRESSION AND FUNCTION OF PORCINE CLASS I MHC GENES IN TRANSGENIC MICE

猪I类主要组织相容性基因已被引入 克隆到C57BL/10小鼠的基因组中。到目前为止，有四只转基因动物 包含不同的I类基因，并且 证明了将转基因传递给后代。一例转基因 LINE在其细胞表面表达SLA抗原，并排斥皮肤 正常C57BL/10小鼠，表明外源SLA抗原是 被认为是一种功能性移植抗原。的表达 这种SLA基因，PD1，与在猪身上观察到的相似， 表明它的表达是受调控的。活体治疗的研究进展 转α、β-干扰素基因可增加PD1的表达 在许多组织中。这一增长与观察到的 内源性移植抗原Kb，但差异显著 来自分化抗原Qa-2。

Porcine class I major histocompatibility genes have been introduced into the genome of C57BL/1O mice. To date, four transgenic animals containing distinct class I genes have been generated, and demonstrated to transmit the transgene to progeny. One transgenic line expresses SLA antigen on its cell surface and rejects skin of normal C57BL/1O mice, indicating that the foreign SLA antigen is recognized as a functional transplantation antigen. Expression of this SLA gene, PD1, parallels that observed in the swine, indicating that its expression is regulated. In vivo treatment of the transgenic with alpha, beta-interferon increases PD1 expression in a number of tissues. This increase parallels that observed for the endogenous transplantation antigen, Kb, but differs markedly from the differentiation antigen, Qa-2.