EFFECT OF DIETARY RESTRICTION ON GENE EXPRESSION

本研究的基本目的是确定饮食对 限制遗传信息在整个生命周期中的传递 一个有机体。 在本研究中，雄性Fischer F344大鼠将在实验室中喂养 随意进食或随意进食的60%（限制饮食）。 平均 并建立这两组大鼠的最大存活率。 周期性地，几个转录因子的翻译和转录活性 将测定来自两种饮食方案的大鼠的组织。 使用 来自脑和肾组织的线粒体后上清液并分离 在肝细胞中，蛋白质合成将通过掺入 将放射性标记的L-缬氨酸溶于酸不溶性物质中，以测定 在本研究中，这些组织的翻译活性。 的 通过测量RNA合成来确定肝脏的转录活性 通过肝细胞的悬浮液 放射性标记的乳清酸不溶性物质。 根据这项研究，它 将有可能确定是否增加寿命与 饮食限制伴随着RNA合成的减少， 蛋白质和/或RNA合成中年龄相关变化的延迟，或 蛋白

The basic objective of this study is to determine what effect dietary restriction has on the transfer of genetic information throughout the life span of an organism. In this study, male Fischer F 344 rats will be fed a laboratory chow diet ad libitum or sixty percent of ad libitum (restricted diet). The mean and maximum survival of these two groups of rats will be established. Periodically, the translational and transcriptional activities of several tissues from rats on the two dietary regimens will be determined. Using the post-mitochondrial supernatant from brain and kidney tissue and isolated hepatocytes, protein synthesis will be measured by the incorporation of radioactively-labeled L-valine into acid-insoluble material to determine the translational activities of these tissues from rats in this study. The transcriptioal activity of liver will be ascertained by measuring RNA synthesis by the ability of suspensions of hepatocytes to incorporate radioactively-labeled orotic acid-insoluble material. From this research, it will be possible to determine whether the increased longevity associated with dietary restriction is accompanied by a decrease in the synthesis of RNA or protein and/or a delay in the age-related changes in the synthesis of RNA or protein.