BIOCHEMICAL BASIS FOR DEFECTIVE DIFFERENTIATION IN GRANULOCYTIC LEUKEMIA

这项工作的主旨是发展生化方法。 粒细胞白血病的早期诊断及治疗方法 诱导白血病细胞发展其部分或全部功能 属性作为部分或完全恢复主机的手段 白血病患者的防御机制。工作的第一个目标是 确定许多生化步骤中的哪一步涉及 正常的粒细胞分化受体液的控制 监管者。结果将与所获得的结果进行比较 来自于对相应阶段白血病细胞的类似研究 以确定成熟度的性质和潜力 阻止的分化步骤的可逆性。生化 对成熟和未成熟的粒细胞进行分析 从血液和骨髓中分离出的外源性药物的作用 细胞调节剂对粒细胞分化的影响，通过 特定细胞成分的合成的变化，是 在先前在此开发的特定培养系统中进行研究 实验室。转化基因之间可能存在的关系 白血病成髓细胞及其调控因素 正常的粒细胞分化正在调查中。

The main thrust of this work is to develop biochemical methods for the early diagnosis of granulocytic leukemia and methods for inducing leukemic cells to develop some or all of their functional properties as a means of partially or completely restoring host defense mechanism in leukemia patients. Work is first aimed at establishing which of the many biochemical steps involved in normal granulocyte differentiation are controlled by humoral regulators. The results will be compared with those obtained from similar studies on leukemic cells at corresponding stages of maturity in order to determine the nature and potential reversibility of the arrested differentiation steps. Biochemical analyses are carried out on mature and immature granulocyte isolated from blood and bone marrow and the effects of external cell regulators on granulocyte differentiation, as measured by changes in the synthesis of specific cellular components, are studied in a defined cultured system previously developed in this laboratory. Possible relationships between transforming genes in leukemic myeloblasts and factors involved in the regulation of normal granulocyte differentiation are under investigation.