ISOLATION OF GENES DIFFERENTALLY EXPRESSED IN BRAIN OF OLD AND YOUNG RATS

尽管有报道称， 在老化的大脑中，老化过程的潜在机制 本身仍然未知。 因此，我们开始了一个新的项目， 旨在探索老年人和年轻人的基因表达差异， 老鼠的大脑 使用减法杂交技术，我们 试图克隆在哺乳动物中差异表达的基因， 老年（24个月）和成熟的年轻（5个月）大鼠。 采用离体垂体碎片 基本的方法是构建两个cDNA文库，一个来自多聚 A+ RNA来自年轻大鼠，另一种来自老年大鼠的poly A+ RNA 大鼠 来自一个年龄组的Poly A+ RNA与cDNA杂交 来自相反年龄组和cDNA/mRNA杂交体 删除. 结果，获得了非杂交cDNA， 随后包装在细菌载体中，应当被富集或 对特定年龄段有很强的特异性 最终，这将 让我们有机会识别和研究那些 与大脑的衰老过程有关， 他们的表现（或相反，他们的相对 在特定的年龄段。

Despite the structural, and biochemical changes reported to occur in aging brain, the mechanism(s) underlying the aging process itself remain unknown. Thus, we have begun a new project designed to explore differential gene expression in old and young rat brain. Using subtraction hybridization techniques we are attempting to clone genes which are differentially expressed in aged (24 month) and mature young (5 month) rats. Briefly, the basic approach is to construct two cDNA libraries; one from poly A+ RNA from young rats, the other from poly A+ RNA from old rats. Poly A+ RNA from one age group is hybridized to the cDNA derived from the opposite age group and the cDNA/mRNA hybrids removed. AS a result, nonhybridizing cDNAs obtained and subsequently package in a bacterial vector, should be enriched or highly specific for a particular age group. Ultimately, this will give us the opportunity to identify and study those genes which are involved (associated) with the aging process in brain by virtue of their increased expression (or conversely, by their relative inactivity) at specific ages.