DEVELOPMENT OF AN ELISA TEST FOR THE DETECTION OF PNEUMOCYSTIS CARINII

肺孢子虫感染的诊断可以利用多种方法 不同的染色程序。这些方法可能非常敏感，但 在性能和性能方面需要高度的复杂性 对测试的解释。此外，这些污渍通常是时间造成的。 耗费时间，包括执行和阅读污点涂片的时间。 目前，酶联免疫吸附试验正被广泛应用于肺炎的诊断。 感染剂。方法已被简化，以便测试 从几分钟到几个小时的表演，不需要技能 解释，因为人们所寻找的是反应中的颜色变化。 我们有4种不同的单抗可以用来进行这个项目。 将尝试将其中一种方法应用于酶联免疫吸附试验，尽管 它将需要广泛的开发工作才能定义最佳 获得最佳灵敏度、灵敏度、快速性和 简单性。

The diagnosis of Pneumocystis infection can be made utilizing a variety of different staining procedures. These methods can be quite sensitive but require a high level of sophistication in the performance and interpretation of the tests. In addition, these stains are generally time consuming, including time to perform and time to read the stained smears. ELISA methodology is being used extensively now in the diagnosis of infectious agents. Methods have been simplified so that the test takes from minutes to a few hours to perform and requires no skill at interpretation since all one looks for is a color change in the reaction. We have 4 different monoclonal antibodies available to pursue this project. Attempts will be made to adapt one of these to an ELISA procedure, although it will take extensive developmental work in order to define the best procedure to obtain optimal sensitivity, sensitivity, rapidity and simplicity.