MUTAGEN FORMATION BY PYROLYSIS OF SOLID FUELS

在此过程中产生的化合物的组成和致突变性 固体燃料的热解将在明确的定义下进行研究 实验条件，以提名更多的化合物 详细的生物学研究和开发化学途径 最令人担忧的化合物的形成。已选择 煤、木材和通常表示部分的模型化合物 在煤和木材中发现的物质将在两种流动装置中进行研究 或床系统在温度范围和时间内运行 在常见的燃烧室类型中遇到。产品将是 分析，与分析中心的核心实验室合作 化学，以区分取代的主要类别， 未被取代的、极性的和含氮的化合物 生物化验。显示出最大生物活性的亚群 将接受更详细的化学分析以确定 主要的诱变剂。导致这些的化学途径 化合物将使用前线分子轨道理论进行评估 并通过选定的模型化合物进行实验。这次会议的重点是 研究对象将是氧和氮化合物，它们普遍存在于 煤和木材不完全燃烧的产物，已知 在有或没有酶激活的情况下引起细菌突变。

The composition and mutagenicity of compounds generated during the pyrolysis of solid fuels will be studied under well-defined experimental conditions in order to nominate compounds for more detailed biological studies and to develop chemical pathways for the formation of the compounds of greatest concern. Selected coals, woods, and model compounds representing moieties commonly found in coal and wood will be studied in either a flow apparatus or bed systems operated over temperature ranges and times encountered in common combustor types. The products will be analyzed, in collaboration with the core laboratory in Analytical Chemistry, to separate out major categories of substituted, unsubstituted, polar, and nitrogen-containing compounds for bioassay. The subgroups showing the greatest biological activity will be subjected to more detailed chemical analyses to identify the major mutagens. The chemical pathways leading to these compounds will be evaluated using frontier molecular orbital theory and by selected experiments with model compounds. The focus of the study will be on oxygen and nitrogen compounds, prevalent in the products of incomplete combustion of coal and wood and known to cause mutation of bacteria with or without enzymatic activation.