DEVELOPMENT OF NANOLITER CAPILLARY LC/MS TECHNIQUES

在质谱分析中的一个长期存在的问题， 和环境样品的绝对水平是分析物 非常低。 解决这个问题的一个方法是开发低容量高容量的 用于质谱仪的通量输送系统。 我们已经承诺， 开发用于纳米级填充毛细管LC和MS的接口。这些 毛细管系统提供了与宽孔LC系统相同的优点， 毛细管GC提供了超过填充柱GC，高通量的分析物进入 MS，但需要显著较低的总分析物水平。 电流 纳米级毛细管LC和毛细管区的发展 共轴连续流快原子电泳 轰击MS和电喷雾电离MS，包括电喷雾 在磁扇区仪器上进行电离。 我们已经应用了这些 分离例如磺胺、喹啉酸的技术 抗生素、大环内酯类抗生素、青霉素、肽类及复合物 蛋白质的混合物。

A perennial problem in the mass spectrometric analysis of both biological and environmental samples is that the absolute level of analyte is extremely low. One approach to this problem is to develop low volume-high flux delivery systems for the mass spectrometer. We have undertaken the development of interfaces for nanoscale packed capillary LC and MS. These capillary systems offer the same advantages over wider-bore LC systems that capillary GC offers over packed-column GC, a high flux of analyte into the MS but with a significantly lower total analyte level necessary. Current developments are in the areas of nanoscale capillary LC and capillary zone electrophoresis interfaced with both coaxial continuous flow fast atom bombardment MS and electrospray ionization MS, including electrospray ionization on a magnetic sector instrument. We have applied these techniques to separations of, for example, sulfonamides, quinolinic acid antibiotics, macrolide antibiotics, penicillins, peptides and complex mixtures of proteins.