THREE-DIMENSIONAL HISTOLOGICAL RECONSTRUCTION

三维结构半自动采集系统 关于组织学材料的信息正在开发中。 系统 应该比使用串行通信的技术更快、更可靠。 部分，虽然分辨率可能有限。 简而言之，嵌入式 将组织块相对于扫描电子显微镜固定 成像系统;块的表面将被成像，并且图像 储存，并连续切片将被删除的内置切片机。 因此，将消除薄切片的处理和配准。 人类和计算机模式识别将转换结果集 将图像转化为三维重建。 的图像 通过这种技术获得的Hermissenda crassicornis与 相同组织的TEM图像。

A semiautomatic system for acquiring three-dimensional structural information about histological material is being developed. The system should be faster and more reliable than techniques that use serial sections, although resolution may be limited. In brief, an embedded tissue block will be fixed relative to a scanning electron microscope imaging system; the surface of the block will be imaged and the image stored, and successive slices will be removed by a built-in microtome. Handling and registration of thin sections will thus be eliminated. Human and computer pattern recognition will transform the resulting set of images into a three-dimensional reconstruction. The images of Hermissenda crassicornis obtained by this technique correlate well with TEM images of the same tissue.