REAL-TIME HIGH-PERFORMANCE CONFOCAL LASER SCANNING OPTICAL MICROSCOPE

一种非移动部件、每秒30帧的激光扫描共焦装置 反射光和荧光显微镜已经研制成功。在……里面 原理上，这项技术可以扩展到透射光 显微镜。全数字控制的声光光束偏转器 电子学，移动512行隔行扫描栅格中的激光束。这个 光线通过荧光光面进入倒置显微镜。 照相机端口，并通过显微镜物镜在物体上成像。 反射光或荧光光通过物镜返回，退出 通过相机端口，并被成像到图像的光电阴极上 解剖管(IDT)。共焦通过对IDT进行栅格扫描来提供 与激光束入射的相干像重合的光圈 在物体上。实时、无抖动的反射和荧光灯 已经制作了各种生物物体的图像。

A no-moving-parts, 30-frames-per-second, laser beam scanning confocal reflected light and fluorescence microscope has been developed. In principle, the technique could be extended to transmission light microscopy. Acousto-optic beam deflectors, controlled by all-digital electronics, move a laser beam in a 512-line, interlaced raster. The light enters an inverted microscope through the epifluorescence side camera port, and is imaged at the object by the microscope objective. Reflected or fluorescent light returns through the objective, exits through the camera port, and is imaged onto the photocathode of an image dissector tube (IDT). Confocality is provided by raster-scanning the IDT aperture coincident with the congruent image of the laser beam incident on the object. Real-time, jitter-free reflected and fluorescent light images of a variety of biological objects have been produced.