RAPID IDENTIFICATION OF MYCOBACTERIAL INFECTIONS USING POLYMERASE CHAIN REACTION

聚合酶链式反应(聚合酶链式反应)设计的快速和 特异地检测出鸟分枝杆菌、胞内分枝杆菌和分枝杆菌。 出现了堪萨斯病毒感染。这些协议使用的是聚合酶链式反应引物。 来源于分枝杆菌16S rRNA基因内的独特序列。 航空分枝杆菌、细胞内分枝杆菌和堪萨斯分枝杆菌的聚合酶链式反应 专门检测1-10个同源基因组DNA的Fentgraph。无聚合酶链反应 对其他18个菌株的DNA也显示出反应性。 分枝杆菌或来自7个密切相关的菌株。此外， 细菌滴定实验表明，1-10个非结核 利用这些聚合酶链式反应可以直接检测到分枝杆菌 协议。目前，这些聚合酶链式反应方法正在被应用于 临床样本的直接分析。

Polymerase chain reaction (PCR) protocols designed to rapidly and specifically detect Mycobacterium avium, M. intracellulare, and M. kansasii infections were developed. These protocols utilize PCR primers derived from unique sequences within the mycobacterial 16s rRNA genes. The M. avium, M. intracellulare, and M. kansasii PCR procedures specifically detect 1-10 fentograms of homologous genomic DNA. No PCR reactivity was demonstrated against DNA from 18 other strains of mycobacteria or from 7 closely related bacterial strains. Furthermore, bacterial titration experiments indicated that 1-10 nontuberculous mycobacterial bacilli could be directly detected using these PCR protocols. Currently, these PCR methodologies are being adapted for direct assays of clinical samples.