MID-ATLANTIC REGIONAL IVEM AND IMAGE ANALYSIS RESOURCE

协调中压输电的地区性资源 电子显微镜(300-400千伏，IVEM)和图像分析 在费城成立，为大西洋中部地区提供服务 国家。预计嵌入的细胞和组织的切片多达 大约5微米厚，甚至更厚的整个坐垫 在这些电压下，可以检查未嵌入的单元。图像将是 记录在胶片上或直接数字化以进行图像分析。这个 图像分析的重点将放在三维分析上。 核心研究将放在样品制备方法的开发上 尤其适用于细胞和组织的电子显微镜研究 这一电压范围，包括发展的染料选择性 特定的细胞组件。图像分析核心研究将定向 朝着开发自动数据采集和高效用户交互的方向 精确提取三维图像的图像处理方法 来自多个图像的信息，包括形态测量信息 结构，要么是连续的显微照片，要么是立体倾斜图像。各种各样的 提出了合作研究项目。这些项目及其 赞助商将与核心研究和开发密切互动 资源能力。意向书和意向用途 已经从纽约、新泽西州、 宾夕法尼亚州、马里兰州和华盛顿特区的培训将在现场进行 当用户来到该设施时，计划为 关于以下能力的额外培训和信息传播 资源。进一步传播成果以及以下能力 该资源将通过由和/或在 资源，并通过发表研究论文和评论论文。 总的目标是带来“最先进的”图像处理 功能与强大的检查工具并列在一起 各种类型和厚度的生物标本 积极和多样化的生物研究环境。

A Regional Resource for Coordinated Intermediate Voltage Transmission Electron Microscopy (300-400 kV, IVEM) and Image Analysis will be established in Philadelphia to serve the mid-Atlantic region of the country. It is expected that sections of embedded cells and tissues up to about 5 micrometers in thickness and even thicker whole mounts of unembedded cells can be examined at these voltages. Images will be recorded either on film or digitized directly for image analysis. The emphasis in the image analysis will be on analysis in three dimensions. Core research will be on development of specimen preparation methods especially suited for study of cells and tissues by electron microscopy in this voltage range, including the development of stains selective for particular cell components. Image analysis core research will be directed toward developing automated data acquisition and efficient user-interactive image manipulation methods for extracting accurate three dimensional information, including morphometric information, from multiple images of structures, either serial micrographs or stereo tilt images. A variety of collaborative research projects is proposed. These projects and their sponsors will interact closely with the core research and the development of Resource capabilities. Letters of interest and intended use of the Resource have been received from users in New York, New Jersey, Pennsylvania, Maryland, and Washington, D.C. Training will be done on-site when users come to the facility, and hands-on workshops are planned for additional training and dissemination of information on the capabilities of the Resource. Further dissemination of results as well as capabilities of the Resource will be done through symposia organized by and/or at the Resource and through publication of research articles and review papers. The overall goal is to bring "state of the art" image processing capabilities into juxtaposition with a powerful tool for examining biological specimens of a wide variety of types and thicknesses in an environment of active and diverse biological research.