RADIOANALYTIC IMAGING SYSTEM

该仪器将被许多研究人员广泛使用， 不同的研究兴趣。 该设备的重要性在于其 广泛适用于目前和未来的NIH受赠者， 在他们的工作中使用放射自显影术或荧光照相术。 大多数研究者 由于其成本，我买不起这种类型的仪器。 此外，授权机构不太可能批准购买 这样的仪器用于单个用户。 因此，这一工具将适合 共享工具RFA的目的。 目前，该工具将用于两个主要领域， 调查：a）它将使我们能够更准确地定量 直接在凝胶上或来自支持物的放射性分布 膜，其中蛋白质已被转移并受到 免疫化学定位B）促进DNA的快速分析 测序、限制性作图、信息定量等。 仪器大大提高了评估的准确性和速度。 参与的NIH赠款跨越不同的调查领域。 它们的范围从胆固醇逆向转运的基础科学研究， 高密度脂蛋白代谢的流行病学筛查研究。 其他领域包括几个涉及分子生物学的项目， DNA和蛋白质的水平。

This instrument will be used by a number of investigators with widely divergent research interests. The importance of this equipment is its broad applicability for current and future NIH grantees who are or will be using autoradiography or fluorography in their work. Most investigators could not afford to purchase this type of instrument due to its costs. Furthermore, granting agencies are not likely to approve the purchase of such an instrument for a single user. Therefore, this instrument would fit the shared instrument RFA purpose. Presently, this instrument will be used for two major areas of investigation: a) It will enable us to more accurately quantitate distribution of radioactivity either directly on gels or from supporting membranes, where protein has been transferred and subjected to immunochemical localization. b) To facilitate rapid analysis of DNA sequencing, restriction mapping, message quantitation, etc. This instrument greatly improves the accuracy and the speed of evaluation. The participating NIH grants span divergent areas of investigation. They range from basic science studies of reverse cholesterol transport and HDL metabolism to large population based epidemiological screening studies. Other areas include several projects involving molecular biology at the level of DNA and proteins.