ACAS 570 ANCHORED CELL ANALYZER AND SORTER

本文要求的ACAS 570交互式激光细胞仪将提供 南亚拉巴马大学医学院， 执行以下功能现在不可用：（1）时间-和 对单个细胞的空间分辨荧光分析，（2） 研究细胞间相互作用的潜力 并直接可视化这种相互作用的影响 使用荧光图像分析，（3）分离锚定细胞， 富集、生长和额外的研究，而不使细胞经历 脱离方案，（4）光漂白后的荧光再分布 为了分析膜组分的胞间和胞内易位， 细胞质结构和生物学相关分子，（5）共聚焦 对细胞和细胞内结构进行成像以精确定位抗原- 或其它探针相关荧光。ACAS 570将成为 为各种分析和制备提供新的能力， 细胞技术在以下NIH资助项目中的应用：（1）内皮细胞 (EC)前列腺素合成和红细胞-EC相互作用，（2）肺 成纤维细胞（LF）异质性和II型肺泡细胞-LF相互作用，（3） II型上皮细胞的肺表面活性物质再循环，（4）催乳素 和分泌颗粒蛋白分布以及相关的细胞内参数 垂体肿瘤细胞模型系统，（5）铁吸收的调节， 单细胞水平，（6）神经突内线粒体移位，（7） 与红细胞镰状化相关的细胞内生化参数 细胞，（8）中性粒细胞中的氧自由基，（9）嗜酸性粒细胞过氧化物酶 调节，（10）聚（ADP-核糖）系统的作用， 化学诱导的β细胞损伤，（11）糖尿病患者的钙转运 心肌病，（12）未成熟心肌中的钠钙交换，（13） 表皮形态发生另外三个项目，不是NIH资助的， （1）钙调蛋白、S100和钙调蛋白的分析 在培养的神经胶质细胞中，（2）钙瞬变的调节， 胃壁细胞，（3）细胞内的特征 神经母细胞瘤细胞系中的螺原体感染。总的来说，这些 项目将在八个不同的部门进行， 医学院，并将代表至少16个不同的实验 解决重要的生物学问题的方法，如果没有 ACAS 570的技术支持。仪器将使 这些和其他研究人员在这个机构探索前沿 科学舞台上最先进的仪器 今天

The ACAS 570 interactive laser cytometer requested herein will provide the University of South Alabama College of Medicine with the capacity to perform the following functions not now available: (1) time- and spatially-resolved fluorescence analyses on individual cells, (2) the potential for studying cell-cell interactions at the level of the individual cells and to directly visualize the effects of such interactions using fluorescence image analysis, (3) isolation of anchored cells for enrichment, outgrowth and additional study without subjecting the cells to detachment protocols, (4) fluorescence redistribution after photobleaching to analyze inter- and intracellular translocation of membrane components, cytoplasmic structures and biologically-relevant molecules, (5) confocal imaging of cells and intracellular structures to precisely locate antigen- or other probe-associated fluorescence. The ACAS 570 will be instrumental in providing new capabilities for a variety of analytical and preparative cell techniques in the following NIH-funded projects: (1) endothelial cell (EC) prostacylin synthesis and red cell-EC interactions, (2) lung fibroblast (LF) heterogeneity and type II alveolar cell-LF interaction, (3) pulmonary surfactant recycling by type II epithelial cells, (4) prolactin and secretogranin distribution and associated intracellular parameters in a pituitary tumor-cell model system, (5) regulation of iron absorption at the single cell level, (6) mitochondria translocation in neurites, (7) intracellular biochemical parameters associated with sickling of red blood cells, (8) oxygen radicals in neutrophils, (9) eosinophil peroxidase regulation, (10) the role of the poly(ADP-ribose) system in chemically-induced beta cell damage, (11) calcium transport in diabetic cardiomyopathies, (12) Na-Ca exchange in immature myocardium, (13) epidermal morphogenesis. Three additional projects, not NIH funded, deal with: (1) an analysis of calcium-modulated proteins, S100 and calmodulin in cultured glial cells, (2) the regulation of calcium transients in gastric parietal cells, and (3) the characterization of intracellular spiroplasma infection in a neuroblastoma cell line. Collectively, these projects will be conducted in eight different departments within the College of Medicine and will represent at least 16 different experimental approaches to important biological problems that cannot be pursued without the technological support of the ACAS 570. The instrumentation will enable these and other investigators at this institution to explore frontier arenas of science with the most sophisticated instrumentation available today.