MASS SPECTROMETRY FACILITY

核心研究将继续侧重于实施和 积分暂态记录仪(ITR)的应用 用于时间阵列检测(TAD)中的时间=飞行(TOF)质量 光谱分析，使其能够检测和集成所有 离子流(在整个质量范围内)从离子加速 这台仪器的来源。这一能力预计将增加 将仪器的全光谱灵敏度提高至少一个 数量级，并提高可用采购率 光谱增加了一个数量级。这两个功能都将具有 对常规GC-MS的深刻影响，特别是随着 重视毛细管柱在络合物分析中的应用 在大多数生物样本中发现的混合物。现有的TOF 仪器已经用连续的信号源和 用于过滤具有反常能量的离子的电子部分。能量 连续源的基本滤波和束脉冲 提高TOF仪器的分辨率。 热辅助FAB Will机理的研究 继续应用这种新的解吸电离技术 用于分析多肽、胆汁酸、卟啉和其他 具有重要生物学意义的分子。合作研究将 重视有机物质代谢谱方法学的推广 尿液中的酸和类固醇用于改变的调查 人类疾病状态的新陈代谢，如糖尿病和 对污染物和药物等外来物质的生理反应。 对服务和协作的请求将需要分析 生理液和其他生化样品中的羧酸 酸、糖、吲哚衍生物等。许多项目涉及 有机合成将继续需要精确的测量来 验证他们产品的经验公式。培训将会 继续是设施方案的一个重要特点； 大约20名研究生接受了详细的培训 通过与五个研究项目的质谱学 设施项目中的专业调查人员。

Core research will continue to focus on the implementation and application of an integrating transient recorder (ITR) which will be used for Time Array Detection (TAD) in time=of-flight (TOF) mass spectrometry to make it possible to detect and integrate all the ion current (over the complete mass range) accelerated from the ion source of this instrument. This capability is expected to increase the full spectrum sensitivity of the instrument by at least an order of magnitude and increase the rate of acquiring useable spectra by an order of magnitude. Both of these features will have a profound effect on conventional GC-MS, especially with increasing emphasis on the use of capillary columns in the analysis of complex mixtures found in most biological samples. An existing TOF instrument has bee" modified with a continuous source and an electric sector to filter out ions with aberrant energy. Energy filtering and beam pulsing of a continuous source substantially increases the resolving power of the TOF instrument. Investigations into the mechanism of thermally assisted FAB will continue as this novel desorption ionization technique is applied to the analysis of peptides, bile acids, porphyrins, and other biologically important molecules. Collaborative research will emphasize extension of metabolic profiling methodology for organic acids and steroids in urine for investigations of altered metabolism in human disease states such as diabetes and of the physiological response to xenobiotics such as pollutants and drugs. Requests for service and collaboration will call for analyses of physiological fluids and other biochemical samples for carboxylic acids, sugars, indole derivatives, etc. Many projects involving organic synthesis will continue to require exact measurements to validate the empirical formulas of their products. Training will continue to be an important feature in the Facility program; approximately 20 graduate students receive detailed training in mass spectrometry through research projects with the five professional investigators in the Facility program.