MODULATION OF IMMUNE FUNCTIONS USING THE IMMUNOTOXIN IL2-PE40

IL-2-PE 40是由白细胞介素-2（IL-2）和PE 40组成的重组嵌合蛋白 （IL-2）和假单胞菌内毒素（PE 40），减去其细胞结合结构域。 这种毒素一旦内化，通过不可逆地抑制 蛋白质合成因此，在其表面上带有IL-2受体的细胞， 是这种毒素的主要目标，可以有效地从 而不会产生太多的不良副作用。 在实验性自身免疫性葡萄膜炎（EAU）中，T细胞起主要作用。一旦 活化后，这些T细胞在其细胞表面上表达IL-2受体， 其可被嵌合毒素靶向并消除。我们有 证明这种毒素能够显著降低 和实验诱导的葡萄膜炎的严重程度。IL-2 PE 40还能够 降低角膜移植排斥反应的发生率和严重程度， 毒素的全身给药。

IL-2-PE40 is a recombinant chimeric protein composed of interleukin-2 (IL-2) and of Pseudomonas endotoxin (PE40), minus its cell-binding domain. This toxin, once internalized, kills cells by irreversibly inhibiting protein synthesis. Hence, cells bearing IL-2 receptors on their surface, which are prime targets for this toxin, can be effectively removed from an organism without producing too many untoward side effects. In experimental autoimmune uveitis (EAU), T cells play a major role. Once activated, these T cells express on their cell surface IL-2 receptors, which can be targeted by the chimeric toxin and eliminated. We have demonstrated that this toxin is able to reduce significantly the incidence and severity of experimentally induced uveitis. IL-2PE40 is also able to reduce the incidence and severity of corneal graft rejection following systemic administration of the toxin.