STUDIES OF HUMAN EPILEPTIC FOCUS

人类癫痫皮层的化学变化的本质是 使用体外切片技术和放射自显影技术进一步研究。 体外切片，添加去甲肾上腺素、谷氨酸、NMDA、 和生长抑素单独或联合使用，以便 确定癫痫灶是否有不同的反应 环核苷酸和肌醇磷酸水平。的响应性 癫痫组织在体外条件下未见明显变化。 不同于周围较少的癫痫组织。PET扫描研究 已经表明Mu阿片受体的结合是轻微的 与非尖峰发作相比，尖峰发作时癫痫皮质的升高 大脑皮层。为了进一步调查这一发现，尖峰和 用选择性小鼠[3-H]-DAGO暴露非棘波癫痫皮层 阿片受体配基和[3-H]-DADLE、a Mu和[3-H]-DADLE的相邻片段 三角洲受体配基。这项研究的主要发现是， 受体位点数或其受体数无显著差异 刺激性皮层与非刺激性皮层的亲和力。的分布情况。 观察到Mu和Delta受体是不同的。在扣球和 在非尖峰皮层，Mu受体以异质性方式与 在1/3和1/2之间距离的两个波段中发现峰层 脑膜和白质。在相同的组织中，Delta受体是 均匀分布，但确实有一个具有最高数字的梯度 位于大脑皮层最外层的受体。数量 此外，我们还研究了棘皮质区与非棘波区皮层中的tcp受体。 使用[3-H]-tcp。研究结果是，没有受体数量或 当比较尖峰组织和非尖峰组织时，亲和力发生变化。 TcP受体在两种组织中的分布相似，并具有 位于最外层的受体数量最多的梯度 大脑皮层的几层。

The nature of the chemical alterations in the human epileptic cortex were investigated further using in vitro slice techniques and autoradiography. The in vitro slices, and the addition of norepinephrine, glutamate, NMDA, and somatostatin were used individually or in combination in order to determine whether the epileptic focus had a different responsiveness at the level of cyclic nucleotides and inositol phosphate. The responsiveness of the epileptic tissue under in vitro conditions was not significantly different from the surrounding less epileptic tissue. PET scan studies have indicated that the binding of the mu opiod receptor was slightly elevated in the spiking epileptic cortex when compared to the nonspiking cortex. To investigate this finding further, sections of the spiking and nonspiking epileptic cortex were exposed to [3-H]-DAGO, a selective mu opiate receptor ligand, and adjacent sections to [3-H)-DADLE, a mu and delta receptor ligand. The major findings of the study are that there are no significant differences for the number of receptor sites or their affinity in the spiking versus nonspiking cortex. The distribution of the mu and delta receptors was observed to be different. In the spiking and nonspiking cortex, the mu receptors were in a heterogeneous manner with the peak layers found in two bands at 1/3 and 1/2 the distance between the meninges and the white matter. In the same tissues, the delta receptor was homogeneously distributed but did have a gradient with the highest number of receptors located in the outermost layer of the cortex. The number of TCP receptors in the spiking versus nonspiking cortex was also investigated using [3-H]-TCP. The findings were that there were no receptor number or affinity changes when the spiking and nonspiking tissues were compared. The distribution of TCP receptors was similar in the two tissues and had a gradient with the highest number of receptors localized in the outermost layers of the cortex.