AUTOMATED CELL COLONY SCANNER SYSTEM

一个微处理器控制的系统已经开发出来扫描组织培养 自动检测并将划线标记放置在该位置 荧光标记的细胞集落。使用荧光素作为标记， 比内在细胞背景荧光低25%的荧光可 被发现。可选择尺寸（例如0.2mm）的小伪影被忽略， 工具。可以设置荧光信号水平阈值（例如70% 高于固有细胞背景）至约15%的准确度内 的内在细胞背景 * 荧光和系统将可靠地发现 信号超过这个水平的菌落。 使用上述典型值， 假阳性的数量通常小于每个培养皿5个 扫描直径80毫米的组织培养皿需要2到4分钟。这 在未来的版本中，通过重新设计 使用垂直而不是倾斜的激光束角的光路 与移动盘的入射消除了两个半的要求 扫描。

A microprocessor-controlled system has been developed to scan tissue culture dishes automatically in order to detect and put scribe marks at the location of fluorescently-labeled cell colonies. Using fluorescein as a,label, fluorescence as low as 25% above intrinsic cell background fluorescence can be detected. Small artifacts of selectable size (e.g. 0.2mm) are ignored by the instrument. The fluorescence signal level threshold can be set (e.g. 70% above intrinsic cell background) to within an accuracy of approximately 15% of intrinsic cell background *fluorescence and the system will reliably find colonies with a signal exceeding that level. With the above typical values, the number of false positives is typically less than 5 per dish and the time to scan an 80mm diameter tissue culture dish is two to four minutes. This time could likely be reduced somewhat in future versions by redesign of the optical path using a perpendicular rather than oblique laser beam angle of incidence with the moving dish eliminating the requirement for two half scans.