300 MHZ NMR INSTRUMENTATION FOR SHARED USE

一台中场(300 MHz)多核核磁共振波谱仪 要求满足Homewood校区用户组的需求 来自约翰霍普金斯大学化学和生物系， 来自医学院的药理学系和 卫生与卫生学院环境健康科学系 公共卫生。一系列化学和生物项目 描述了意义：(1)黄曲霉毒素的生物合成研究， 环境真菌毒素、β-内酰胺类和糖肽类 抗生素。(2)1α，25-二羟基维生素D3(骨化三醇)的合成 和氟化类似物。(3)合成和结构研究 低聚糖、糖肽和糖脂的研究 碳水化合物识别和肝脏受体结合研究。(4) 核磁共振法鉴定核磁共振标记位点 ~(13)C富集酶活化抑制α-胰凝乳酶 抑制剂。硅形成的低温核磁共振研究 离子。化学发光和生物发光反应机理的研究。(5) 受阻和变形的烯烃的氧化，环状反应 将研究偏振光和卡宾重排。(6) 前列腺素及其氮杂类似物的合成。(7)对中国文化的研究 人胎盘的作用机制及抑制剂的设计 芳香酶和羊毛甾醇14α-脱甲基酶。(8)准备一份 用于受体结合研究的各种放射性药物。

An intermediate field (300 MHz), multinuclear NMR spectrometer is requested to meet the needs of a user group at the Homewood campus of The Johns Hopkins University from the Departments of Chemistry and Biology, and from the Department of Pharmacology in the School of Medicine and the Department of Environmental Health Sciences in the School of Hygiene and Public Health. A range of projects of chemical and biological significance is described: (1) Biosynthetic studies of aflatoxins, environmental mycotoxins, and of beta-lactam and glycopeptide antibiotics. (2) Syntheses of 1alpha,25-dihydroxyvitamin D3 (calcitriol) and fluorinated analogues. (3) Synthetic and structural investigations of oligosaccarides, glycopeptides and glycolipids for research in carbohydrate recognition and hepatic receptor binding studies. (4) Identification by NMR techniques of the labeled sites of alpha-chymotrypsin inhibited with 13C-enriched enzyme-activated inhibitors. Study by low-temperature NMR of the formation of silicenium ions. Examination of chemi- and bioluminescent reaction mechanisms. (5) Oxygenation of hindered and deformed olefins, the reactions of circularly polarized light and carbene rearrangements will be investigated. (6) Synthesis of prostaglandins and their aza-analogues. (7) Studies of the mechanism of action and design of inhibitors for human placental aromatase and lanosterol 14alpha-demethylase. (8) Preparation of a variety of radiopharmaceuticals for receptor binding studies.