MONOCLONAL ANTIBODIES IN THE DIAGNOSIS OF PNEUMOCYSTIS PNEUMONIA

目前诊断感染的方法学 卡氏肺孢子虫(PC)依靠示范或生物 具有典型的形态，使用特殊的染色，如 甲胺-银、甲苯胺蓝-O等 都是具体的，它们都必须谨慎地解释，因为 类似酵母的有机体或细胞材料有时可以模仿 PC的出现。用抗体进行特异性组织学染色 直接针对PC是不可能的，因为缺乏 适当的抗体。我们中的一人(J.K.)已经产生了单克隆 针对人PC的抗体制剂。这些 抗体被用来开发一种特异的荧光抗体。 染色用于开发一种特异性荧光抗体染色 可用于肺炎的辅助诊断。支气管灌洗 样品用传统的甲苯胺蓝-O平行测试 染色和用单抗间接免疫荧光 请继续。共收集了93例患者的126份标本。 为PC染色。其中45人(36%)在以下时间呈阳性 IFA检测甲苯胺蓝O(TBO)阳性43例(34%)。这些 两种方法的符合率为98%，没有错误 IFA检测阳性2例，假阴性2例。这些结果表明， 使用单抗的IFA可以提供一种简单、快速、 微生物实验室诊断肺炎的灵敏方法。

Current methodology for the diagnosis of infection with Pneumocystis carinii (PC) relies on demonstration or organisms with typical morphology using special stains such as methanamine-silver, toluidine blue-O, etc. None of these stains are specific and they all must be interpreted with caution since yeast-like organisms or cellular materials can sometimes mimic the appearance of PC. Specific histologic staining using antibody directed against PC has not been possible due to the lack of appropriate antibody. One of us (J.K.) has produced monoclonal antibody preparations directed against human PC. These antibodies were used to develop a specific fluorescent antibody stain to be used to develop a specific fluorescent antibody stain to be used to aid in the diagnosis of PC pneumonia. Bronchial lavage specimens were tested in parallel by traditional toluidine blue-O stain and by a monoclonal antibody indirect immunofluorescence proceduce. A total of 126 specimens from 93 patients were stained for PC. Fortyfive of these (36%) were positive by toluidine blue O (TBO) and 43 (34%) were positive by IFA. These was a 98% agreement between both methods with no false positives and 2 false negatives by IFA. These results show that IFA using monoclonal antibodies can provide a simple, fast, yet sensitive method for diagnosing PC by microbiology laboratories.