EVALUATION OF HORSE BLOOD AGAR (HBA) SUPPLEMENTED WITH PYRIDOXAL

一种裂解-离心式血液培养方法的使用促使 美国将探索使用补充血液琼脂培养基来 允许营养缺乏的链球菌(NDS)生长 它们不会生长在传统的血液或巧克力琼脂上。这些 NDS可引起心内膜炎和菌血症，因此 侦测到。如果能开发出一种合适的介质，除了 作为血液培养介质，它也可以被更多地使用 常规用于脓肿和其他无菌液体培养， 场合也可能包含NDS作为重要病原体。我们的 传统的马血琼脂配方中添加了 不同浓度的PXL初始试验和对照试验 NDS菌株，以确定最低浓度 PXL是获得NDS良好生长所必需的。此HBA+PXL 然后用35种不同的细菌菌株进行测试 检查是否有生长抑制或显著变化 PXL可能导致的形态学改变。这些测试表明 HBA和HBA+PXL之间的可比增长。除了……之外 这35种、296株A组链球菌也是 由于之前的一份报告曾建议 PXL对A组链球菌抑制作用的可能性我们 没有发现抑制，事实上发现可能的分离物生长 在PXL存在的情况下更好。含0.0005%PXL的糖化血红蛋白现在 在我们实验室用作两种血液的常规培养基 培养以及其他标本，如伤口、脓肿和 无菌体液。我们已经有两个NDS的血液分离株， 它的识别是通过使用这种介质来促进的，因为 以及至少一个颈部伤口分离株被判定为 具有重要的临床意义。

The use of a lysis-centrifugation blood culture method prompted us to explore the use of a supplemented blood agar medium to permit the growth of nutritionally deficient streptococci (NDS) which do not grow on traditional blood or chocolate agars. These NDS can cause endocarditis and bacteremia so are important to detect. If a suitable medium could be developed, in addition to use as a blood culture medium, it could also be used more routinely for abscesses and other sterile fluid cultures, which on occasion may also contain NDS as significant pathogens. Our traditional formula of horse blood agar was supplemented with differing concentrations of PXL initially and tested with control strains of NDS in order to determine the lowest concentration of PXL necessary to obtain good growth of NDS. This HBA + PXL was then tested with 35 strains of a wide variety of bacteria to check for either inhibition of growth or significant changes in morphology that the PXL might result in. These tests showed comparable growth between HBA and HBA + PXL. In addition to these 35 species, 296 strains of Group A streptococci were also tested in parallel due to a previous report which had suggested the possibility of inhibition of Group A streptococci by PXL. We found no inhibition and in fact found that may isolates grew better in the presence of PXL. HBA with 0.0005% PXL is now used as a routine plating medium in our laboratory for both blood cultures as well as other specimens, such as wounds, abscesses and sterile body fluids. We have had 2 blood isolates of NDS, the recognition of which was facilitated by the use of this medium, as well as at least one neck wound isolate which was judged to be clinically significant.