MOLECULAR GENETIC EVENTS IN LUNG CANCER

工作概要 A. 34例小细胞肺癌标本 研究了患者的myc家族DNA扩增。 没有一 在11例未经治疗的患者的肿瘤标本中， 任何myc家族的DNA扩增。23个肿瘤中的6个 从化疗患者中获得的标本 myc家族的DNA扩增（4个N-myc，2个L-myc） B。 26例肿瘤标本和正常组织， 来自不同的小细胞肺癌患者， NCI-Navy Medical Oncology分支。 制备DNA， 用适当的限制性内切酶消化，和 与检测限制性片段长度的4个探针杂交 DNA多态性（RFLP）。 25例至少有一个等位基因为杂合子。 的DNA 肿瘤组织中有一个杂合等位基因缺失 25名患者中的23名。 2例例外中有1例患者 肺外小细胞肺癌 从这些RFLP 我们的研究得出结论，3 p等位基因的缺失是存在的， 在小细胞肺癌中很常见， 小细胞肺3号染色体短臂缺失 癌

Summary of Work A. Thirty-four tumor specimens from small cell lung cancer patients were studied for myc family DNA amplification. None of the 11 tumor specimens obtained from untreated patients had DNA amplification of any of myc family. Six of 23 tumor specimens obtained from chemotherapy treated patients had DNA amplification of the myc family (4 N-myc, 2 L-myc) B. Twenty-six tumor specimens and normal tissues were obtained from different small cell lung cancer patient treated at the NCI-Navy Medical Oncology Branch. DNA was prepared, digested with appropriate restriction enclonucleases, and hybridized to 4 probes which detect restriction fragment length polymorphisms (RFLP) in DNA. Twenty-five were heterozygous for at least one allele. The DNA from tumors showed deletion of one of the heterozygous alleles in 23 of the 25 patients. One of the 2 exceptions was a patient with extrapulmonary small cell lung cancer. From these RFLP studies we concluded the deletion of 3p alleles is present and common in small cell lung cancer providing additional evidence for deletion of the short arm of chromosome 3 in small cell lung cancer.